The present invention relates to a biological enzymatic production of silkworm pupa oil and extraction method of alpha linolenic acid, which comprises the following steps: (1): drying and crushing, crushing and sieving to dried silkworm pupa powder; (2) aqueous enzymatic: in accordance with the mass ratio of material to water 1:4~7 and water mixed pupa powder evenly, then two step enzymolyze: first, according to the quality of silkworm pupa powder ratio of 1.0~2.0% to 30~40 DEG C, adding pepsin, enzymatic 2~4h hydrolysis process control pH value of 1.0~2.0, then according to the quality of silkworm chrysalis powder ratio of 1.0~2.0% adding papain, 55~65 C enzyme 2~4h, enzymatic hydrolysis process control pH 6.0~7.0, after the completion of the enzyme solution was heated to 80~85 DEG C inactivated enzyme; (3) by centrifugation, silkworm oil water separation; (4) chrysalis oil fatty acid preparation; (5): 70~75% urea entraing purity linolenic acid. The method is applied to the silkworm pupa cell by biological enzyme method and urea embedding method, and has the advantages of simple process, safety, environmental protection, low cost and good quality of silkworm chrysalis oil and alpha linolenic acid.
【技术实现步骤摘要】
【技术保护点】
生物酶法生产蚕蛹油并提取α?亚麻酸的方法，其特征在于：所述方法包括下列步骤：（1）烘干粉碎：蚕蛹经烘干粉碎过筛后，得到蚕蛹粉备用；（2）水相酶解：按照料水质量比1:4~7将蚕蛹粉与水混合均匀，然后分两步进行酶解：首先，按照蚕蛹粉质量1.0~2.0%的比例加入胃蛋白酶，于30~40℃酶解2~4h，酶解过程中控制pH值为1.0~2.0，然后按照蚕蛹粉质量1.0~2.0%的比例加入木瓜蛋白酶，于55~65℃酶解2~4h，酶解过程中控制pH为6.0~7.0，酶解完成后酶解液加热至80~85℃灭酶；（3）离心、油水分离：将酶解液离心分离得到残渣和上清液，上清液加热至50~60℃并静置进行物理破乳，然后将上清液进一步通过离心分离得到蚕蛹毛油和蚕蛹多肽液；（4）蚕蛹毛油精制：向蚕蛹毛油中加入2~4倍蚕蛹毛油体积的稀碱溶液，于30~40℃条件下搅拌1~3h，静置一段时间，待油水分离后，去除稀碱液，得到蚕蛹精油；（5）蚕蛹油脂肪酸制备：蚕蛹精油与1~1.2mol/L的氢氧化钠溶液按体积比1:3~5混合，于60~70℃小搅拌反应2~4h，静置冷却至常温，加水至澄清透明，调pH至2~3，加入蚕蛹精油3倍体积...
【技术特征摘要】
【专利技术属性】
技术研发人员：陈贵才，常艺海，侯保兵，王丽，聂月美，王贤玉，崔志文，丁瑜，
申请(专利权)人：浙江汇能动物药品有限公司，甘肃汇能生物工程有限公司，
类型：发明
国别省市：
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