The invention discloses a method for inducing culture of axillary buds of sugarcane seedling process induced by cultured in axillary buds of sugarcane seedlings, using tissue as seed seedling substrate moisture germination. Through the method of humidity control tissue, inhibit the growth of fungi and bacteria, preventing the growth of axillary buds of sugarcane to the base, to ensure the cutting seedlings in the process of axillary buds of sugarcane sugarcane seedling base surrounding axillary buds of fungi and bacteria, to reduce sugar cane axillary bud as explants cultured seedlings of the pollution rate, thereby improving the axillary buds of sugarcane seedlings inoculated the success rate of. Experiments show that the paper towel wet, can reach the purpose of promoting sugarcane bud germination; at the same time according to the wet and dry conditions of sugarcane seedling induced axillary bud tissue process management and control can be obtained, good quality and good seedling emergence explants were inoculated with low contamination rate of axillary buds of sugarcane seedlings. In a word, the technical requirement of the management personnel is low, the production cost is reduced, and the implementation is easy to popularize.
【技术实现步骤摘要】
本专利技术属于甘蔗种苗快繁生产
,尤其涉及一种甘蔗腋芽苗诱导培养方法。
技术介绍
甘蔗为禾本科甘蔗属,主要用于生产蔗糖,一般采用种茎种植或组培的方法扩繁种苗。在甘蔗组培苗培育过程中,需获取甘蔗腋芽萌发形成的甘蔗腋芽苗作为外植体进行组培扩繁,而在甘蔗腋芽苗诱导培养的过程中,一般采用椰糠、泥炭土、蛭石等种子育苗基质进行保湿催芽,但是使用这些基质会导致水分不容易控制,出现甘蔗腋芽苗生长不整齐、质量较差,基质易附着在甘蔗腋芽苗基部,而大量的真菌和细菌会使后期对甘蔗腋芽苗灭菌不彻底造成污染,浪费材料,提高生产成本。
技术实现思路
本专利技术要解决的技术问题是提供一种甘蔗腋芽苗诱导培养方法,该法管理简单、出苗整齐质量好,还可抑制真菌和细菌的生长,从而降低甘蔗腋芽苗外植体接种时的污染率,提高甘蔗组培过程中甘蔗腋芽苗接种的成功率。为解决上述技术问题本专利技术采用以下技术方案:甘蔗腋芽苗诱导培养方法,在甘蔗腋芽苗诱导培养的过程中,采用纸巾作为种子育苗基质进行保湿催芽。将纸巾包裹住甘蔗茎段进行保湿。纸巾包裹住带腋芽的甘蔗茎段1~5层。包裹住的甘蔗茎段置于恒温培养箱中培养。包裹住的甘蔗茎段呈水平摆放,腋芽向上放入底部镂空的托盘中,然后将托盘置于恒温培养箱中。培养的温度为38℃,培养时间为5~10天。纸巾用喷壶喷水将纸巾喷湿。喷水每天2~4次。甘蔗茎段喷施1~2次1~10mg/L的赤霉素。甘蔗茎段喷施1~2次800~1000倍液的甲基托布津或多菌灵。针对现有甘蔗组培苗培育中存在的问题,专利技术人建立了一种甘蔗腋芽苗诱导培养方法,在甘蔗腋芽苗诱导培养的过程中,采用纸巾作为种子育 ...
【技术保护点】
一种甘蔗腋芽苗诱导培养方法,其特征在于在甘蔗腋芽苗诱导培养的过程中,采用纸巾作为种子育苗基质进行保湿催芽。
【技术特征摘要】
1.一种甘蔗腋芽苗诱导培养方法,其特征在于在甘蔗腋芽苗诱导培养的过程中,采用纸巾作为种子育苗基质进行保湿催芽。2.根据权利要求1所述的甘蔗腋芽苗诱导培养方法,其特征在于将所述纸巾包裹住甘蔗茎段进行保湿。3.根据权利要求2所述的甘蔗腋芽苗诱导培养方法,其特征在于:所述纸巾包裹住带腋芽的甘蔗茎段1~5层。4.根据权利要求3所述的甘蔗腋芽苗诱导培养方法,其特征在于:所述包裹住的甘蔗茎段置于恒温培养箱中培养。5.根据权利要求4所述的甘蔗腋芽苗诱导培养方法,其特征在于:所述包裹住的甘蔗茎段呈水平摆放,腋芽向上放入底部镂空的托盘中,然后将托盘...
【专利技术属性】
技术研发人员:罗晟昇,何洪良,蒋亚琴,韦海球,江清梅,刘连军,陈海生,唐利球,马文清,黄珍玲,廖韦卫,秦昌鲜,彭崇,闭德金,莫周美,郭强,
申请(专利权)人:广西南亚热带农业科学研究所,
类型:发明
国别省市:广西;45
还没有人留言评论。发表了对其他浏览者有用的留言会获得科技券。