用于孕妇外周血样的保存液和孕妇外周血样的保存方法技术

技术编号:15525483 阅读:96 留言:0更新日期:2017-06-04 13:44
本申请公开了一种用于孕妇外周血样的保存液和孕妇外周血样的保存方法。本申请的保存液由阿氏液和细胞凋亡抑制剂组成,阿氏液:细胞凋亡抑制剂的体积比为5‑15:0.03‑0.1。本申请的保存液率先将阿氏液和细胞凋亡抑制剂联合应用于孕妇外周血液的保存,能有效的延缓孕妇外周血液中细胞的凋亡、维持细胞的各项指标,使样品尽量保持新鲜;并且可以维持细胞形态、为细胞提供养分、保持细胞各项理化性;为后续的有核红细胞和其它稀有细胞的梯度离心和流式分选奠定基础,大量新鲜的有核红细胞的保有,为非侵入性产前诊断的准确性提供了保障。

Preservation solution for peripheral blood sample of pregnant woman and method for preserving peripheral blood sample of pregnant woman

The present invention discloses a preservation solution for peripheral blood samples of pregnant women and a method for preserving peripheral blood samples of pregnant women. The application of preservation solution consists of a fluid and apoptosis inhibitor, apoptosis inhibitor alsever's solution: the volume ratio of 5 15:0.03 0.1. The application of the first solution will save alsever's solution and apoptosis inhibitor in maternal peripheral blood, can effectively delay the apoptosis of the peripheral blood cells of pregnant women to maintain the index of cells, the samples keep fresh; and can maintain cell shape, provide nutrients, for cells to maintain the physical cell chemical properties; lay the foundation for the subsequent centrifugation of nucleated red blood cells and other rare cells and flow cytometry, a large number of fresh keeping of nuclear red blood cells, the accuracy of non-invasive prenatal diagnosis provides a guarantee.

【技术实现步骤摘要】
【国外来华专利技术】PCT国内申请,说明书已公开。

【技术保护点】
PCT国内申请,权利要求书已公开。

【技术特征摘要】
【国外来华专利技术】PCT国内申请,...

【专利技术属性】
技术研发人员:邓乐潘健昌刘萍
申请(专利权)人:深圳华大基因科技有限公司
类型:发明
国别省市:广东,44

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