The invention discloses a high-throughput DNA synthetic product detection method, comprising the following steps: multiple DNA components were inserted into cloning vector and transferred to the screening of strains, screened clones connected with DNA components; respectively the number of clones, and develop the corresponding cloning broth; the same number of the selected clones liquid mixing and Plasmid Extraction; connection sequencing labels, constructing a hybrid sequencing library, in which different mixed sequencing library sequencing tags are different; by high-throughput sequencing, sequence of DNA components; compared with the reference sequence, with DNA synthesis target DNA assembly accuracy. The method of the invention adopts a hybrid library construction strategy to apply high throughput sequencing technology to the detection of DNA chip synthesis products, thereby reducing the product detection and the screening cost of the correct product.
【技术实现步骤摘要】
【国外来华专利技术】PCT国内申请，说明书已公开。
【技术保护点】
PCT国内申请，权利要求书已公开。
【技术特征摘要】
【国外来华专利技术】PCT国内申请，...
【专利技术属性】
技术研发人员：康康，陈世宏，沈玥，王云，徐讯，
申请(专利权)人：深圳华大基因研究院，
类型：发明
国别省市：广东,44
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