The present invention provides a method for forecasting the susceptibility to coronary heart disease and reagent box, the method steps: 1. extraction human genomic DNA of peripheral white blood cells; 2. for subjects of DDAH1 polymorphism in 396D / I, namely SEQ ID sequence table 1 shows, the sequence table SEQ and ID 2 3 the F and R sequence specific oligonucleotide primers, amplified from the DNA, and through Taqman probe sequence sequence table SEQ ID 4 and 5 are shown to detect different genotypes of DDAH1 gene 396 loci; 3. subjects of individual DDAH1 gene loci 396 the I / I, D / I and D / D genotype by non conditional Logisti C regression model was used to predict the susceptibility to coronary heart disease. The present invention utilizes probes to specifically combine genotypes of different polymorphic loci to detect different genotypes.
【技术实现步骤摘要】
【技术保护点】
１．一种预测冠心病易感性的方法，具体步骤是：ａ．提取人外周血白细胞的基因组ＤＮＡ；ｂ．对于受试者个体中ＤＤＡＨ１基因多态性位点－３９６ Ｄ／Ｉ，即序列表ＳＥＱ ＩＤ １所示，通过序列表ＳＥＱ ＩＤ ２和３所示的Ｆ和Ｒ寡核苷酸引物序列，特异性地从上述ＤＮＡ中扩增出来，并通过序列表ＳＥＱ ＩＤ ４和５所示的Ｔａｑｍａｎ探针碱基序列来实现对ＤＤＡＨ１基因－３９６位点不同的基因型的检测；ｃ．将受试者个体的ＤＤＡＨ１基因－３９６位点的Ｉ／Ｉ，Ｄ／Ｉ和Ｄ／Ｄ基因型引入非条件Ｌｏｇｉｓｔｉｃ回归分析模型中来预测冠心病的发病易感性。
【技术特征摘要】
【专利技术属性】
技术研发人员：汪道文，丁虎，吴斌，
申请(专利权)人：华中科技大学同济医学院附属同济医院，
类型：发明
国别省市：83[]
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