重组葡萄球菌蛋白Ａ基因、含有该基因的表达载体及用途制造技术

本发明专利技术公开了一种重组葡萄球菌蛋白Ａ基因及其用途。本发明专利技术重组葡萄球菌蛋白Ａ基因的核苷酸序列为ＳＥＱ　ＩＤ　ＮＯ：１所示，所编码的蛋白质氨基酸序列为ＳＥＱ　ＩＤ　ＮＯ：２所示。本发明专利技术将葡萄球菌蛋白Ａ与小泛素融合得到了重组葡萄球菌蛋白Ａ基因，所表达的重组蛋白既利于蛋白的提纯又增加了蛋白的稳定性和活性，经实验证明，本发明专利技术所表达的重组葡萄球菌蛋白Ａ与天然葡萄球菌蛋白Ａ的生物活性无明显区别。本发明专利技术重组葡萄球菌蛋白表达和纯化方法具有生产时间短、表达效率高、表达量大、易于纯化等优点。

Recombinant staphylococcal protein A gene, expression vector containing the gene and use thereof

The invention discloses a recombinant staphylococcal protein A gene and uses thereof. The nucleotide sequence of the recombinant staphylococcal protein A gene of the invention is SEQ, ID, NO:1, and the amino acid sequence of the encoded protein is SEQ, ID, NO:2. The invention of staphylococcal protein A and small ubiquitin fusion by recombinant staphylococcal protein A gene, recombinant protein expression and purification is helpful to increase the stability and activity of proteins, the experiment proved that there was no significant difference between A recombinant staphylococcal protein A and natural staphylococcal protein expressed by the invention of biological activity. The recombinant staphylococcal protein expression and purification method of the invention has the advantages of short production time, high expression efficiency, large expression quantity and easy purification.

【技术实现步骤摘要】

【技术保护点】
１．一种重组葡萄球菌蛋白Ａ基因，其特征在于：其核苷酸序列为ＳＥＱ　ＩＤ　ＮＯ：１所示。

【技术特征摘要】

【专利技术属性】
技术研发人员：李德山，高学慧，任桂萍，王文飞，刘铭瑶，
申请(专利权)人：哈尔滨市哈科隆生物制药研究所，
类型：发明
国别省市：93[中国|哈尔滨]