Agrobacterium mediated method to cultivate a field of biotechnology by Changchun flowers of transgenic plants, which comprises the following steps: obtaining sterile seedlings of periwinkle, hypocotyl of sterile seedlings; preparation of Agrobacterium bacteria, using Agrobacterium tumefaciens mediated transformation Changchun flower hypocotyls from hypocotyl; and culture medium on callus induction, callus was inoculated into; the callus differentiation culture medium, green callus; green callus was inoculated into adventitious bud induction culture medium, to take adventitious buds; adventitious buds, rooting in the culture medium, too Changchun flowers of transgenic plants. The invention establishes a stable Agrobacterium mediated genetic transformation system of Changchun flowers, laid the foundation for further study on network regulation of metabolic pathway and improve the content of TIAs can be further applied to genetic engineering breeding and production of metabolites of TIAs in.
【技术实现步骤摘要】
【技术保护点】
一种培育根癌农杆菌介导的长春花转基因植株的方法，其特征在于，包括如下步骤： 步骤一，取长春花无菌苗，获取无菌苗的下胚轴； 步骤二，制备根癌农杆菌菌液，利用根癌农杆菌介导长春花下胚轴的转化； 步骤三，取下胚轴，置于愈伤组织诱导培养基上进行培养，得愈伤组织； 步骤四，将愈伤组织接种到分化培养基上进行培养，得绿色愈伤组织； 步骤五，将绿色愈伤组织接种到不定芽诱导培养基上进行培养，得不定芽； 步骤六，取不定芽，置入生根培养基中进行培养，得长春花转基因植株。
【技术特征摘要】
【专利技术属性】
技术研发人员：唐克轩，王荃，潘琪芳，袁芳，王国丰，刑世海，赵静雅，
申请(专利权)人：上海交通大学，
类型：发明
国别省市：31[中国|上海]
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