The present invention provides a method for cultivating and producing PRRS vaccine sensitive cells, which comprises the following steps: 1) with microcarrier culture sensitive cells in the bioreactor; 2) in microcarrier culture the sensitive cells inoculated with porcine reproductive and respiratory syndrome virus; 3) to cultivate the sensitive cells 4); harvest virus liquid, PRRS vaccine production. The method uses microcarrier culture techniques to culture sensitive cells in a bioreactor to produce blue ear vaccine. Because the micro carrier provides greater surface area, the cells can be grown to high cell density, and the automatic control bioreactor culture environment parameters, makes the growth and reproduction of the virus cells in the superior environment, improve the quality of the vaccine virus titer, more stable.
【技术实现步骤摘要】
【技术保护点】
一种培养敏感细胞生产蓝耳病疫苗的方法,其包括以下步骤: 1)用微载体培养敏感细胞; 2)在微载体培养的所述敏感细胞上接种猪繁殖与呼吸综合征病毒; 3)继续培养所述敏感细胞; 4)收获病毒液,生产蓝耳病疫苗。
【技术特征摘要】
【专利技术属性】
技术研发人员:陈文庆,刘俊生,王建超,
申请(专利权)人:北京天和瑞生物科技有限公司,
类型:发明
国别省市:11[中国|北京]
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