检测猪萨佩罗病毒的引物、Taqman-MGB探针及试剂盒制造技术

本发明专利技术公开了检测猪萨佩罗病毒的引物、Taqman-MGB探针及试剂盒，属于动物病原微生物检测技术领域。用于检测猪萨佩罗病毒的引物核苷酸序列如SEQIDNO:1~2所示，Taqman-MGB探针的DNA序列如SEQIDNO:3所示。本发明专利技术的引物和Taqman-MGB探针特异性高，稳定性好，检测准确率高，Taqman-MGB诊断试剂盒具有检测快速、灵敏性高、稳定性好、易于定量、假阳性低等特点，有利于规模化和自动化检测分析。采用Taqman-MGB实时荧光定量RT-PCR方法，克服了病毒分离操作过程繁琐且不易鉴定和传统PCR检测速度慢、易污染、扩增后需电泳检测和每次检测的样品数量少等缺点。

Pig zapero virus detection primer, Taqman-MGB probe and kit

Taqman-MGB primer, probe and kit of the invention discloses a detection zapero swine virus, which belongs to the technical field of animal pathogenic microorganism detection. For the detection of porcine zapero virus primer nucleotide sequence as shown in SEQIDNO:1~2, the DNA sequence of Taqman-MGB probe is shown in SEQIDNO:3. The primers and Taqman-MGB probe specificity of the invention is high, good stability, high detection accuracy, Taqman-MGB diagnosis kit has the advantages of rapid detection, high sensitivity, good stability, easy quantitative, low false positive, facilitates the analysis of scale and automatic detection. By using the method of Taqman-MGB real-time fluorescence quantitative RT-PCR, to overcome the small number of virus isolation and the operation process is complicated and not easy to identify and traditional PCR slow detection speed and easy pollution, amplified after electrophoresis and each test sample defect.

【技术实现步骤摘要】

【技术保护点】
一种检测猪萨佩罗病毒的引物，其特征在于，核苷酸序列如SEQ?ID?NO:1~2所示。

【技术特征摘要】

【专利技术属性】
技术研发人员：陈俊伟，
申请(专利权)人：广东温氏食品集团股份有限公司，
类型：发明
国别省市：