一种乙酸耐性乙醇生产酿酒酵母菌株及菌株筛选方法技术

一种乙酸耐性乙醇生产酿酒酵母菌株，该菌株分类学命名为酿酒酵母（Ｓａｃｃｈａｒｏｍｙｃｅｓ　ｃｅｒｅｖｉｓｉａｅ）Ｅ５．９，保藏号为：ＣＧＭＣＣ　Ｎｏ．３６１８。筛选方法为液体全筛选法，步骤如下：１）菌株诱变：对酿酒酵母出发菌株进行物理、化学或生物诱变；２）液体全筛选：将所有诱变菌株平均转接于２４孔板中，首先无选择压力预培养，然后添加选择压力继续培养至多个孔出现正突变体；３）固体平板筛选：稀释正突变培养物，涂布固体选择培养基，挑选优势抗性克隆；４）传代驯化：将优势抗性克隆在选择性液体培养基中传代驯化，筛选遗传稳定的抗逆菌株。

Acetic acid tolerant ethanol producing Saccharomyces cerevisiae strain and screening method of strain

An acetic acid tolerance ethanol production of Saccharomyces cerevisiae strains, the strain taxonomy named Saccharomyces cerevisiae (Saccharomyces cerevisiae) E5.9, No.3618 storage number: CGMCC. Screening method for wet screening method, the steps are as follows: 1) mutant strains of Saccharomyces cerevisiae strain: the physical, chemical or biological mutation; 2) liquid screening: all the mutant strains all transferred to the average of 24 Kong Banzhong, the first non selective pressure pre culture, then add to selection pressure to culture hole positive mutant 3); solid plate screening: dilution positive mutant cultures, coated solid culture medium, selection of dominant resistant clones; 4) were domesticated: advantage of resistant clones in selective liquid medium were domesticated, sieve resistant strains were selected by genetic stability.

【技术实现步骤摘要】

【技术保护点】
１．一种乙酸耐性乙醇生产酿酒酵母菌株，该菌株分类学命名为酿酒酵母（Ｓａｃｃｈａｒｏｍｙｃｅｓ　ｃｅｒｅｖｉｓｉａｅ）Ｅ５．９，保藏号为：ＣＧＭＣＣ　Ｎｏ．３６１８。

【技术特征摘要】

【专利技术属性】
技术研发人员：王士安，袁博，李福利，
申请(专利权)人：中国科学院青岛生物能源与过程研究所，
类型：发明
国别省市：95[中国|青岛]