猪细小病毒灭活疫苗的制备方法及其产品技术

本发明专利技术公开了猪细小病毒灭活疫苗的制备方法，包括：(1)将猪细小病毒弱毒株接种ST细胞，培养得到生产种毒；(2)将处理后的微载体加入到生物反应器中的细胞生长液中，搅拌；(3)将ST细胞接入到生物反应器中的微载体中进行细胞的繁殖培养；(4)将猪细小病毒生产种毒感染生物反应器中悬浮培养的ST细胞进行病毒的繁殖培养；(5)收获繁殖的病毒液，灭活，配苗，即得。本发明专利技术方法细胞维持时间长，细胞活力高、活细胞密度大，有利于病毒的持续繁殖，所制备的病毒液病毒滴度高。本发明专利技术方法的生产过程衔接紧密、顺畅，规模放大容易，猪细小病毒的收获方便，质量稳定，可显著降低生产成本、有效提高疫苗产量和质量。

Preparation method of inactivated vaccine of porcine parvovirus and products thereof

Including the method of the present invention discloses the preparation of porcine parvovirus inactivated vaccine: (1) the porcine parvovirus strain was inoculated to ST cells cultured for virus production; (2) the microcarriers treated into a stirred bioreactor to cell growth in the medium; (3) ST access to the cells in the bioreactor in microcarrier cell reproduction culture; (4) the production suspension culture of porcine parvovirus virus infection in the bioreactor ST cells for virus breeding; (5) the virus liquid inactivated with harvest breeding, seedlings, i.e.. The method of the invention has the advantages of long maintenance time, high cell viability and large living cell density, and is favorable for the continuous reproduction of the virus. The method of the invention has the advantages of close and smooth production process, easy enlargement of scale, convenient harvesting of the pig parvovirus, stable quality, low production cost, and improved vaccine yield and quality.

【技术实现步骤摘要】

【技术保护点】
一种猪细小病毒灭活疫苗的制备方法，包括：（１）将猪细小病毒（Ｐｏｒｃｉｎｅｐａｒｖｏ　ｖｉｕｒｓ）弱毒株接种ＳＴ细胞，培养得到生产种毒；（２）将处理后的微载体加入到生物反应器的细胞生长液中，搅拌；（３）将ＳＴ细胞接入到生物反应器的微载体中，搅拌，进行细胞的繁殖培养；（４）将猪细小病毒生产种毒感染生物反应器中悬浮培养的ＳＴ细胞，进行病毒的繁殖培养；（５）收获繁殖的病毒液，灭活，配苗，即得。

【技术特征摘要】

【专利技术属性】
技术研发人员：姜力，藏玉婷，柴华，王彬，王琪，任德强，戴秀莉，张智明，武佳斌，张明艳，张婷婷，
申请(专利权)人：哈药集团生物疫苗有限公司，
类型：发明
国别省市：93