一种抗生素的高产菌株及其制备方法和用途技术

本发明专利技术公开了一种抗生素的高产菌株及其制备方法和用途。所述的高产菌株是属于Ｇｌａｒｅａ?ｌｏｚｏｙｅｎｓｉｓ的诱变菌株，以ＣＧＭＣＣ?２９３３的保藏编号保藏于中国微生物菌种保藏管理委员会普通微生物中心。通过下述步骤制备得到：（ａ）将保藏号为ＡＴＣＣ２０９５７的种子液和亚硝基胍混合，得到混合液ａ；（ｂ）将混合液ａ和破壁酶混合，得到原生质体；（ｃ）原生质体再生，得到单菌落；和（ｄ）培养单菌落，得到诱变菌株。所得菌株遗传稳定性好，产量性状稳定，发酵生产的杂质少，适合于工业化生产。

Antibiotic high-yield strain and preparation method and application thereof

The invention discloses a high-yield antibiotic strain, a preparation method and an application thereof. The high yield strains belonged to Glarea mutation strains, lozoyensis? CGMCC? 2933 is preserved in the preservation number of bacterial China microbe preservation management committee. Prepared by the following steps: (a) the preservation number of ATCC20957 seed mixture and NTG, obtain a mixed solution a; (b) the mixture of a and lytic enzyme mixture by protoplast; protoplast regeneration (c), single colony; and (d) single colony was cultured, get mutant strain. The obtained strains have good genetic stability, stable yield characters and less impurities in fermentation, and are suitable for industrial production.

【技术实现步骤摘要】

【技术保护点】
一种Ｇｌａｒｅａ　ｌｏｚｏｙｅｎｓｉｓ的诱变菌株，以ＣＧＭＣＣ　２９３３的保藏编号保藏于中国微生物菌种保藏管理委员会普通微生物中心。

【技术特征摘要】

【专利技术属性】
技术研发人员：徐晶，陈懿，季晓铭，高霄梁，刘石东，张兆利，
申请(专利权)人：上海天伟生物制药有限公司，
类型：发明
国别省市：31[中国|上海]