The invention discloses a colloidal detection method for nitrofuran veterinary drugs, including AOZ, AMOZ, AHD, SEM colloidal gold immunochromatographic strip for rapid detection of furazolidone metabolite, AMOZ, furantoin metabolite and furacilin metabolite, and is applied to visual detection of nitrofuran veterinary drug metabolites in animal-derived food. The optimum conditions for AOZ colloidal gold strip of furazolidone metabolite were as follows: NC membrane (cellulose nitrate membrane) was Millipore HF90s; colloidal gold particle size was 20 nm; the optimum working pH of gold-labeled antibody was 9; the amount of antibody used in gold-labeled antibody was 20 ug; the dilution ratio of sheep anti-mouse second antibody was 10 times; the dilution ratio of gold-labeled antibody was 8 times; the concentration of coated original was 0.4 mg/mL; the coated original and antibody were diluted with PB dilution solution; the second antibody and the coated antibody The original spraying amount is 0.3 ULL/cm; NC film does not need to be sealed. The detection limit of AOZ colloidal gold strip for furazolidone metabolite is 0.5 ug/kg (measured by NPAOZ) and the detection time is about 1 Omin. The present invention provides a method for the determination of nitrofuran veterinary drug colloids. The raw materials used in this method are cheap and easy to obtain, the route is simple, and the total yield is high.
【技术实现步骤摘要】
一种硝基呋喃类兽药胶体检测方法
本专利技术属于生物与新医药
，具体涉及兽药检测领域。
技术介绍
兽药在防治动物疾病、提高生产效率、改善畜产品质量等方面起着十分重要的作用。然而，由于养殖人员对科学知识的缺乏以及一味地追求经济利益，致使滥用兽药现象在当前畜牧业中普遍存在。随着人们对动物源食品由需求型向质量型的转变，动物源食品中的兽药残留已逐渐成为全世界关注的一个焦点。兽药残留是指用药后蓄积或存留于畜禽机体或产品(如鸡蛋、奶品、肉品等)中原型药物或其代谢产物，包括与兽药有关的杂质残留。目前，在动物源食品中较容易引起兽药残留量超标的兽药主要有抗生素类、磺胺类和激素类等药物。
技术实现思路
本专利技术的目的在于提供一种硝基呋喃类兽药胶体检测方法，呋喃唑酮代谢物AOZ、呋喃它酮代谢物AMOZ、呋喃妥因代谢物AHD、呋喃西林代谢物SEM胶体金免疫层析试纸条快速检测方法,并应用于动物源性食品中硝基呋喃类兽药代谢物的可视化检测。呋喃唑酮代谢物AOZ胶体金试纸条的最适条件为:NC膜(硝酸纤维素膜)为MilliporeHF90s;胶体金粒径20nm;金标抗体最适工作pH为9;金标抗体...
【技术保护点】
1.一种硝基呋喃类兽药胶体检测方法，呋喃唑酮代谢物AOZ、呋喃它酮代谢物AMOZ、呋喃妥因代谢物AHD、呋喃西林代谢物SEM胶体金免疫层析试纸条快速检测方法,并应用于动物源性食品中硝基呋喃类兽药代谢物的可视化检测，呋喃唑酮代谢物AOZ胶体金试纸条的最适条件为:NC膜(硝酸纤维素膜)为MilliporeHF90s;胶体金粒径20nm;金标抗体最适工作pH为9;金标抗体中抗体使用量为20μg;羊抗鼠二抗稀释倍数10倍;金标抗体稀释倍数8倍;包被原浓度0.4 mg/mL;包被原与抗体均用PB稀释液稀释;划膜时二抗和包被原喷涂量0.3μL/cm;NC膜不需要封闭处理，呋喃唑酮代谢物...
【技术特征摘要】
1.一种硝基呋喃类兽药胶体检测方法，呋喃唑酮代谢物AOZ、呋喃它酮代谢物AMOZ、呋喃妥因代谢物AHD、呋喃西林代谢物SEM胶体金免疫层析试纸条快速检测方法,并应用于动物源性食品中硝基呋喃类兽药代谢物的可视化检测，呋喃唑酮代谢物AOZ胶体金试纸条的最适条件为:NC膜(硝酸纤维素膜)为MilliporeHF90s;胶体金粒径20nm;金标抗体最适工作pH为9;金标抗体中抗体使用量为20μg;羊抗鼠二抗稀释倍数10倍;金标抗体稀释倍数8倍;包被原浓度0.4mg/mL;包被原...
【专利技术属性】
技术研发人员：凌青云，
申请(专利权)人：江苏威凌生化科技有限公司，
类型：发明
国别省市：江苏,32
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