The invention discloses an antidote for fish microcystin and a method for using the antidote, including the total flavonoids of Astragalus membranaceus and glutathione independently packaged. The total mass fraction of total flavonoids and glutathione in Astragalus was 100 (150): (60 90). The total flavonoids of Astragalus membranaceus were dissolved in alcohol solution A, glutathione in water solution B and vegetable oil as solution C. The solution A was sprayed evenly on the feed surface, and the total flavonoids of Astragalus membranaceus membranaceus were sprayed 100 150 mg per kilogram of feed. 90 mg glutathione; spraying solution C evenly on the feed surface; feeding the feed prepared by the above process to the fish poisoned by microcystin continuously for 3 to 5 days as a course of treatment. The invention has the advantages of simple and convenient use, non-toxic side effects, no drug resistance, no pollution to water environment, effective removal of ROS in fish body, induction of GST expression of detoxification gene, significant alleviation of fish microcystin poisoning symptoms, and provides new drugs and methods for the prevention and treatment of fish microcystin poisoning.
【技术实现步骤摘要】
一种鱼类微囊藻毒素解毒剂及使用方法
本专利技术涉及鱼类环境毒素解毒剂及使用方法,尤其是涉及一种鱼类微囊藻毒素解毒剂及使用方法。
技术介绍
近年来鱼类养殖水体中蓝藻水华频繁爆发,导致水中微囊藻毒素(Microcystins,MCs)含量增加。微囊藻毒素是微囊藻、鱼腥藻、束丝藻、颤藻等养殖水体中常见有毒蓝藻产生的单环七肽类毒素,具有强烈的肝毒性、促癌活性和肾毒性,对淡水鱼养殖造成了极大的危害,自然界中常见MC-LR、MC-RR、MC-YR三种异构体。养殖水体中的食用鱼类会通过鳃、口和上皮细胞等途径摄取水体中的微囊藻毒素,主要积累到肝脏和肾脏等组织中。微囊藻毒素经典致毒机制是不可逆地抑制肝脏细胞中蛋白磷酸酶1(proteinphosphatase1,PP1)和2A(proteinphosphatase2A,PP2A)的活性,导致蛋白质的过磷酸化,同时微囊藻毒素可引起鱼体内活性氧(Reactiveoxygenspecies,ROS)的大量产生,从而对DNA造成氧化损伤,并可以激活细胞内核酸内切酶,造成DNA链的无规律断裂。目前的微囊藻毒素的防治技术多集中在降低或去除水体中的蓝藻或微囊藻毒素,包括:使用机械法清除蓝藻水华(裴毅,黄维,陈飞勇,等,2009)、使用活性炭吸附法去除水中微囊藻毒素(李莉,袁倩倩,李倩,等,2017)、使用化学药剂氧化法去除水中蓝藻(潘海祥,楼小平,邵王芳,2013)、利用微囊藻毒素降解菌降解微囊藻毒素MC-LR(CN201710782159.7)、利用碳纳米管去除水中微囊藻毒素(CN200310101678.0)、利用辐照降解水体中微囊藻 ...
【技术保护点】
1.一种鱼类微囊藻毒素解毒剂,其特征在于,包括独立包装的黄芪总黄酮和谷胱甘肽。
【技术特征摘要】
1.一种鱼类微囊藻毒素解毒剂,其特征在于,包括独立包装的黄芪总黄酮和谷胱甘肽。2.根据权利要求1所述鱼类微囊藻毒素解毒剂,其特征在于,黄芪总黄酮和谷胱甘肽的质量份数比为(100-150):(60-90)。3.如权利要求1所述鱼类微囊藻毒素解毒剂的使用方法,其特征在于,包括以下步骤:(1)将黄芪总黄酮溶于酒精溶液制成溶液A,谷胱甘肽溶解于水制成溶液B,植物油作为溶液C;(2)将溶液A均匀喷洒于...
【专利技术属性】
技术研发人员:毕相东,董少杰,杨广,
申请(专利权)人:天津农学院,
类型:发明
国别省市:天津,12
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