The utility model discloses a fluorescence quantitative PCR system, including the heat conduction base and a fluorescence detector array is used for the heat conducting seat tube placed in the tank, the heat conducting seat is arranged at the top of the bracket is provided with a sample sample pool, pool and hole bracket pipe groove corresponding to the sealing cover plate is arranged above the tube, and two symmetrical sides seal the cover plate is provided with a flange and the bracket are hinged on both sides of the sample pool and retaining flange with the heat conducting seat is provided with a plurality of embedded electric heating plate, a heat conducting seat is arranged at the bottom of the heat radiating block, block is provided with a tube groove communicated with the through hole, both sides of the heat dissipating block is provided with a heat pipe, the heat dissipating block is provided with a positioning screw. The fluorescence detector is fixed on the sliding block positioning screw on. By setting multiple electric heating sheets, the utility model realizes the rapid rising temperature of the heat conducting seat, and realizes the rapid cooling of the heat conducting seat with the heat dissipation block and the heat pipe. The fluorescent detector adopts the bottom mounted mode, and the detection accuracy can be effectively improved by reasonably controlling the thickness of the heat dissipation block.
【技术实现步骤摘要】
一种荧光定量PCR系统
本技术属于荧光分析
,具体涉及一种荧光定量PCR系统。
技术介绍
PCR(聚合酶链式反应)检测系统的基本原理类似于DNA的天然复制过程,其特异性依赖于与靶序列两端互补的寡核苷酸引物,目前已广泛应用于分子生物学研究、临床诊断等领域中。但现有的PCR检测系统大都存在升降温过程缓慢的问题,不利于突发性传染病的检测,同时,受加热模块的位置影响,荧光检测器通常采用顶部或侧部检测的方式,但检测过程中的干扰因素较多,如管盖水汽、光纤易磨损断裂等等,难以达到检测的稳定性和精度要求。
技术实现思路
本技术所要解决的技术问题是提供一种操作简单,稳定性好,检测精度较高的荧光定量PCR系统。为解决上述技术问题,本技术采用的技术方案是:一种荧光定量PCR系统,包括导热座和荧光检测器,导热座上阵列排布有用于放置试管的管槽,导热座的顶部设有样品池托架,样品池托架上设有与管槽一一对应的圆孔,圆孔的直径小于试管管口的外径,试管的上方设有封盖板,封盖板的两侧对称设有凸缘,样品池托架的两侧铰接有与凸缘配合的扣环,导热座内嵌装有多个电热片,导热座的底部设有散热块,散热块上设有与管槽 ...
【技术保护点】
一种荧光定量PCR系统,其特征在于:包括导热座和荧光检测器,所述导热座上阵列排布有用于放置试管的管槽,所述导热座的顶部设有样品池托架,所述样品池托架上设有与管槽一一对应的圆孔,所述圆孔的直径小于试管管口的外径,所述试管的上方设有封盖板,所述封盖板的两侧对称设有凸缘,所述样品池托架的两侧铰接有与凸缘配合的扣环,所述导热座内嵌装有多个电热片,所述导热座的底部设有散热块,所述散热块上设有与管槽连通的通孔,所述散热块的两侧设有热管,所述散热块的下方设有定位丝杆,所述荧光检测器为从内向外排列的多个,且均固定在定位丝杆的滑块上,所述定位丝杆通过步进电机驱动。
【技术特征摘要】
1.一种荧光定量PCR系统,其特征在于:包括导热座和荧光检测器,所述导热座上阵列排布有用于放置试管的管槽,所述导热座的顶部设有样品池托架,所述样品池托架上设有与管槽一一对应的圆孔,所述圆孔的直径小于试管管口的外径,所述试管的上方设有封盖板,所述封盖板的两侧对称设有凸缘,所述样品池托架的两侧铰接有与凸缘配合的扣环,所述导热座内嵌装有多个电热片,所述导热座的底部设有散热块,所述散热块上设有与管槽连通的通孔,所述散热块的两侧设有热管,所述散热块的下方设有定位丝杆,所述荧光检测器为从内向外排列...
【专利技术属性】
技术研发人员:徐沁,
申请(专利权)人:海门中科基因生物科技有限公司,
类型:新型
国别省市:江苏,32
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