The invention provides a detection kit for a super gonorrhea strain and a detection method thereof. The kit contains 6 amino acids expressed in SEQ ID NO.1 to SEQ ID NO.6. The detection method includes steps: (1) separating the samples by separating liquid and suspending the isolated cells in the serum-free medium; (2) the amino acid sequences of the cells after the resuspension are reincubated with the 6 peptides shown by SEQ ID NO.1 ~ SEQ ID NO.6, and the highly specific anti human IgG in the co incubation system is added to the system. The antibody reacted with the antigen antibody; (3) the system after the reaction of the original antibody was washed, the chromogenic substrate was added to the chromogenic reaction, and the number of spots generated by the reaction was counted. The kit of the invention can be fully synthesized artificially, the production is convenient and fast, the stability and specificity are high, and the operation of the kit is easy, and the cost is greatly reduced.
【技术实现步骤摘要】
一种超级淋病菌株的检测试剂盒及其检测方法
本专利技术属于医疗检测
,具体涉及一种超级淋病菌株的检测试剂盒及其检测方法。
技术介绍
超级淋病菌株病也称“H041”,是一种新型的由基因突变或者人犬泛交造成质粒交换而成的淋病双球菌,该细菌对全部目前应用的抗生素产生抗药性。2011年首次发现于日本,美国和挪威等地也相继出现感染者。2013年,美国卫生部警告称该细菌的传染性可能高于艾滋病。H041病毒的侵略性相当强,能在短时间内感染更多的人,感染者可能会在短短几天内出现脓毒性休克症状,甚至死亡。超级淋病菌株可以通过抗原与抗体的特异性结合,根据循环外周血中超级淋病菌株的抗体的数量,可判断是否感染超级淋病菌株,用于超级淋病菌株感染的临床辅助诊断,可以快速、敏感的检测出人体的感染情况。目前还没有比较有效的专门针对超级淋病菌株的精确检测手段。
技术实现思路
有鉴于此,本专利技术的目的在于提供一种超级淋病菌株的检测试剂盒及其检测方法,以期解决上述现有技术中存在的至少部分技术问题。为了实现上述目的,本专利技术提供一种超级淋病菌株的检测试剂盒,其包括氨基酸序列为SEQIDNO.1~SEQIDNO.6所示的6条多肽,分别为:SEQIDNO.1:GluGluGlyAlaGluAlaLeuAlaAlaAlaValLeuAlaGlyIleGlnGlySerThrAla;SEQIDNO.2:GlnGluLeuGluSerGluAlaGlyGlyThrAlaSerIleArgValAlaArgThrGlyGly;SEQIDNO.3:ThrMetGlyAlaArgArgGluGlyIleGl ...
【技术保护点】
1.一种超级淋病菌株的检测试剂盒,其特征在于,其包括氨基酸序列为SEQ ID NO.1~SEQ ID NO.6所示的6条多肽:SEQ ID NO.1:Glu Glu Gly Ala Glu Ala Leu Ala Ala Ala Val Leu Ala Gly Ile Gln Gly Ser Thr Ala;SEQ ID NO.2:Gln Glu Leu Glu Ser Glu Ala Gly Gly Thr Ala Ser Ile Arg Val Ala Arg Thr Gly Gly;SEQ ID NO.3:Thr Met Gly Ala Arg Arg Glu Gly Ile Glu Thr Ala Ala Pro Ala Thr Ala Thr Gly Ala;SEQ ID NO.4:Glu Ala Met Asp Leu Leu Ala Thr Ile Ala Ser Thr Ser Glu Ala Glu Gly Gly Ala Ala;SEQ ID NO.5:Gly Leu Thr Lys Ala Gly Ala Ile Val Gly Glu Gly Ser Leu A ...
【技术特征摘要】
1.一种超级淋病菌株的检测试剂盒,其特征在于,其包括氨基酸序列为SEQIDNO.1~SEQIDNO.6所示的6条多肽:SEQIDNO.1:GluGluGlyAlaGluAlaLeuAlaAlaAlaValLeuAlaGlyIleGlnGlySerThrAla;SEQIDNO.2:GlnGluLeuGluSerGluAlaGlyGlyThrAlaSerIleArgValAlaArgThrGlyGly;SEQIDNO.3:ThrMetGlyAlaArgArgGluGlyIleGluThrAlaAlaProAlaThrAlaThrGlyAla;SEQIDNO.4:GluAlaMetAspLeuLeuAlaThrIleAlaSerThrSerGluAlaGluGlyGlyAlaAla;SEQIDNO.5:GlyLeuThrLysAlaGlyAlaIleValGlyGluGlySerLeuAlaThrAlaIleGlyGlu;SEQIDNO.6:ThrAlaIleGluGlyAspLeuGluAlaSerAlaThrGlyAlaMetSerAlaIleGlyAla。2.根据权利要求1所述的超级淋病菌株的检测试剂盒,其特征在于,在所述超级淋...
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