The present invention provides a method for preparing can induce antitumor immunity by alginate microparticles, using genetic engineering to construct MIP-3 eukaryotic expression plasmid and transformed into alpha tumor cells, obtained by screening stable expression of MIP-3 tumor cells. At the same time, cultivation of Escherichia coli and soluble alginate solution, by high temperature high pressure disinfection after MIP-3 alpha tumor cells mixed in bacterial alginate solution, then using the atomizer to \MIP-3 alpha tumor cells bacterial alginate solution was atomized, and atomized particles sprayed into the calcium chloride solution, through the gauze filter can be obtained by injection of MIP-3 alpha tumor cell bacterial alginate microparticles, MIP-3 alpha tumor cell bacterial alginate micro particles in the environment can be expressed and secreted MIP-3 protein in vitro. The micro particles were injected into the mouse lumen and proved to be effective in inhibiting tumor growth and metastasis in tumor models.
【技术实现步骤摘要】
【技术保护点】
１．一种可以诱导肿瘤免疫反应的海藻酸盐微颗粒的制备方法，其特征在于：包括以下步骤：（１）、构建ＭＩＰ－３α真核表达质粒并将其转染肿瘤细胞；（２）、通过Ｇ４１８筛选获得可以稳定表达ＭＩＰ－３α的肿瘤细胞株，称为ＭＩＰ－３α肿瘤细胞；（３）、培养大肠杆菌，将大肠杆菌溶于质量浓度为１．５％的海藻酸盐溶液，获得细菌海藻酸盐溶液；（４）、将步骤（２）的ＭＩＰ－３α肿瘤细胞混匀于经高温高压消毒后的步骤（３）的细菌海藻酸盐溶液中，此时获得“ＭＩＰ－３α肿瘤细胞细菌海藻酸盐溶液”；（５）、通过雾化器将步骤（４）的“ＭＩＰ－３α肿瘤细胞细菌海藻酸盐溶液”进行雾化，并将雾化颗粒喷入２５０ｍＭｏ...
【技术特征摘要】
【专利技术属性】
技术研发人员：谭光宏，黄风迎，黎岳南，郭峻丽，黄用豪，王华，
申请(专利权)人：海南医学院，
类型：发明
国别省市：66
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