纳豆芽孢杆菌谷氨酸脱氢酶编码基因、蛋白及菌株制造技术

本发明专利技术涉及生物技术领域的纳豆芽孢杆菌谷氨酸脱氢酶（ＧＤＨ）编码基因、蛋白及菌株。纳豆芽孢杆菌谷氨酸脱氢酶编码基因具有如ＳＥＱ？ＩＤ？ＮＯ：１所示的碱基序列；该基因编码的蛋白质具有如ＳＥＱ？ＩＤ？ＮＯ：２所示的氨基酸序列；本发明专利技术还提供了一种纳豆芽孢杆菌（Ｂａｃｉｌｌｕｓ？Ｎａｔｔｏ）ＳＪＴＵ１－４ＣＧＭＣＣ？ＮＯ．２８０１。本发明专利技术确定了纳豆芽孢杆菌的谷氨酸脱氢酶编码序列，即ＲｏｃＧ基因，为将来研究酶的活性位点和通过定点突变改变发酵菌株ＧＤＨ酶活从而生产出低氨纳豆奠定了基础，ＲｏｃＧ基因缺失菌株的氨产量可降低５０％。

Bacillus natto glutamate dehydrogenase coding gene, protein and strain

The invention relates to Bacillus natto glutamate dehydrogenase (GDH) coding gene, protein and strain in the field of biotechnology. The genes encoding glutamate dehydrogenase of Bacillus natto are like SEQ? ID? The nucleotide sequence shown in NO:1; the protein encoded by the gene is like SEQ? ID? The present invention also provides a Bacillus subtilis NO:2 (Bacillus) Natto) SJTU14CGMCC? NO.2801. The present invention determines glutamate dehydrogenase encoding sequence of Bacillus natto, RocG gene, to study enzyme active sites and by point mutation changes the strain GDH enzyme to produce low ammonia natto foundation, ammonia production of RocG gene mutant strain can be reduced by 50%.

【技术实现步骤摘要】

【技术保护点】
一种纳豆芽孢杆菌谷氨酸脱氢酶编码基因，其特征在于，其序列如ＳＥＱ　ＩＤ　ＮＯ：１所示。

【技术特征摘要】

【专利技术属性】
技术研发人员：张建华，于湘莉，陈丽丽，刘斌，
申请(专利权)人：上海交通大学，
类型：发明
国别省市：31[中国|上海]