基于外泌体核酸进行微生物检测的方法及其应用技术

本发明专利技术提供了基于外泌体核酸进行微生物检测的方法及其应用。所述方法包括：(a)从待测样品中分离获得外泌体核酸，所述待测样品来自于第一物种；(b)对所述外泌体核酸进行测序，获得测序结果；(c)从所述测序结果中排除对应于所述第一物种的核酸序列，从而获得经去除处理的序列数据；和(d)将所述经去除处理的序列数据与微生物数据库的核酸序列进行比对，获得所述样品中的微生物检测结果。

Method and application of microbiological detection based on exosomal nucleic acid

The invention provides a method for microbiological detection based on exosome nucleic acid and its application. The method comprises: (a) separating and obtaining exosomal nucleic acid from the sample to be tested, the sample to be tested is from the first species; (b) sequencing the exosomal nucleic acid to obtain the sequencing result; (c) excluding the nuclear acid sequence corresponding to the first species from the sequencing result to obtain the sequence data after being removed; and (d) comparing the sequence data after being removed with The nucleic acid sequence of the microorganism database is compared to obtain the microorganism detection result in the sample.

【技术实现步骤摘要】
【国外来华专利技术】PCT国内申请，说明书已公开。

【技术保护点】
PCT国内申请，权利要求书已公开。/n

【技术特征摘要】
【国外来华专利技术】20170418...

【专利技术属性】
技术研发人员：卢森，高雅，姬敬开，麻锦敏，赵佳，黄国栋，陈芳，蒋慧，
申请(专利权)人：深圳华大生命科学研究院，
类型：发明
国别省市：广东;44