杀虫基因和使用方法技术

提供了具有杀虫活性的组合物及其使用方法。所述组合物包括具有杀虫活性的分离的和重组的多肽，编码所述多肽的重组和合成的核酸分子，包含所述核酸分子的DNA构建体和载体，包含所述载体的宿主细胞和针对所述多肽的抗体。编码所述多肽的核苷酸序列可以用于DNA构建体或表达盒中，用于在目的生物中的转化和表达。提供的组合物和方法可用于生产具有增强的害虫抗性或耐受性的生物。还提供了包含编码本发明专利技术的杀虫蛋白质的核苷酸序列的转基因植物和种子。这种植物对昆虫和其他害虫具有抗性。提供了用于产生本文公开的各种多肽以及使用这些多肽控制或杀死害虫的方法。还包括用于检测样品中的本发明专利技术多肽的方法和试剂盒。

Insecticidal genes and methods of use

【技术实现步骤摘要】
【国外来华专利技术】杀虫基因和使用方法领域本专利技术涉及用于防治害虫，特别是植物害虫的方法和组合物。相关申请的交叉引用本申请要求于2016年9月6日提交的美国申请NO.62/383,773、2016年9月9日提交的美国申请NO.62/385,441、2017年1月20日提交的美国申请NO.62/448,410和2017年3月27日提交的美国申请NO.62/477,036的权益，这些申请的内容通过引用整体并入本文。经由EFS-WEB作为文本文件提交的序列表的提及序列表的官方拷贝作为ASCII格式化序列表经由EFS-Web以电子方式提交，文件名为AGB038PCT-SEQLISTING-1059491.TXT，于2017年9月6日创建，具有1,001,165比特的大小，且与说明书同时提交。包含在该ASCII格式化文件中的序列表是说明书的一部分，并且通过引用整体并入本文。背景害虫、植物疾病和杂草对作物可以是严重的威胁。由于害虫和疾病的损失据估计占全世界农业生产的37％，其中由于昆虫、细菌和其他生物的占13％。毒素是在微生物致病性和/或宿主免疫应答逃避中起重要作用的毒力决定簇。来自革兰氏阳性细菌芽孢杆菌属(Bac本文档来自技高网...

【技术保护点】
1.一种具有杀虫活性的重组多肽，其包含：(a)包含与选自SEQ ID NO：1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85,86,87,88,89,90,9...

【技术特征摘要】
【国外来华专利技术】2016.09.06 US 62/383,773;2016.09.09 US 62/385,441;1.一种具有杀虫活性的重组多肽，其包含：(a)包含与选自SEQIDNO：1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85,86,87,88,89,90,91,92,93,94,95,96,97,98,99,100,101,102,103,104,105,106,107,108,109,110,111,112,113,114,115,116,117,118,119,120,121,122,123,124,125,126,127,128,129,130,131,132,133,134,135,136,137,138,139,140,141,142,143,144,145,146,147,148,149,150,151,152,153,154,155,156,157,158,159,160,161,162,163,164,165,166,167,168,169,170,171,172,173,174,175,176,177,178,179,180,181,182,183,184,185,186,187,188,189,190,191,192,193,194,195,196,197,198,199,200,201,202,203,204,205,206,207,208,209,210,211,212,213,214,215,216,217,218,218,219,220,221,222,223,224,225,226,227,228,229,230,231,232,233,234,235,236,237,238,239,240,241,242,243,244,245,246,247,248,249,250,251,252,253,254,255,256,257,258,259,260,261,262,263,264,265,266,267,268,269,270,271,272,273,274,275,276,277,278,279,280,281,282,283,284,285,286,287,288,289,290,291,292,293,294,295,296,297,298,299,300,301,302,303,304,305,306,307,308或309中所示序列的氨基酸序列具有至少90％序列同一性百分比的氨基酸序列的多肽；或者(b)包含SEQIDNO:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85,86,87,88,89,90,91,92,93,94,95,96,97,98,99,100,101,102,103,104,105,106,107,108,109,110,111,112,113,114,115,116,117,118,119,120,121,122,123,124,125,126,127,128,129,130,131,132,133,134,135,136,137,138,139,140,141,142,143,144,145,146,147,148,149,150,151,152,153,154,155,156,157,158,159,160,161,162,163,164,165,166,167,168,169,170,171,172,173,174,175,176,177,178,179,180,181,182,183,184,185,186,187,188,189,190,191,192,193,194,195,196,197,198,199,200,201,202,203,204,205,206,207,208,209,210,211,212,213,214,215,216,217,218,218,219,220,221,222,223,224,225,226,227,228,229,230,231,232,233,234,235,236,237,238,239,240,241,242,243,244,245,246,247,248,249,250,251,252,253,254,255,256,257,258,259,260,261,262,263,264,265,266,267,268,269,270,271,272,273,274,275,276,277,278,279,280,281,282,283,284,285,286,287,288,289,290,291,292,293,294,295,296,297,298,299,300,301,302,303,304,305,306,307,308或309中所示的氨基酸序列的多肽。2.权利要求1的多肽，所述多肽进一步包含异源氨基酸序列。3.一种组合物，其包含权利要求1或2的多肽。4.一种编码氨基酸序列的重组核酸分子，所述氨基酸序列包含：(a)与选自SEQIDNO:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85,86,87,88,89,90,91,92,93,94,95,96,97,98,99,100,101,102,103,104,105,106,107,108,109,110,111,112,113,114,115,116,117,118,119,120,121,122,123,124,125,126,127,128,129,130,131,132,133,134,135,136,137,138,139,140,141,142,143,144,145,146,147,148,149,150,151,152,153,154,155,156,157,158,159,160,161,162,163,164,165,166,167,168,169,170,171,172,173,174,175,176,177,178,179,180,181,182,183,184,185,186,187,188,189,190,191,192,193,194,195,196,197,198,199,200,201,202,203,204,205,206,207,208,209,210,211,212,213,214,215,216,217,218,218,219,220,221,222,223,224,225,226,227,228,229,230,231,232,233,234,235,236,237,238,239,240,241,242,243,244,245,246,247,248,249,250,251,252,253,254,255,256,257,258,259,260,261,262,263,264,265,266,267,268,269,270,271,272,273,274,275,276,277,278,279,280,281,282,283,284,285,286,287,288,289,290,291,292,293,294,295,296,297,298,299,300,301,302,303,304,305,306,307,308或309中所示序列的氨基酸序列具有至少90％的序列同一性百分比；或者(b)SEQIDNO:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85,86,87,88,89,90,91,92,93,94,95,96,97,98,99,100,101,102,103,104,105,106,107,108,109,110,111,112,113,114,115,116,117,118,119,120,121,122,123,124,125,126,127,128,129,130,131,132,133,134,135,136,137,138,139,140,141,142,143,144,145,146,147,148,149,150,151,152,153,154,155,156,157,158,159,160,161,162,163,164,165,166,167,168,169,170,171,172,173,174,175,176,177,178,179,180,181,182,183,184,185,186,187,188,189,190,191,192,193,194,195,196,197,198,199,200,201,202,203,204,205,206,207,208,209,210,211,212,213,214,215,216,217,218,218,219,220,221,222,223,224,225,226,227,228,229,230,231,232,233,234,235,236,237,238,239,240,241,242,243,244,245,246,247,248,249,250,251,252,253,254,255,256,257,258,259,260,261,262,263,264,265,266,267,268,269,270,271,272,273,274,275,276,277,278,279,280,281,282,283,284,285,286,287,288,289,290,291,292,293,294,295,296,297,298,299,300,301,302,303,304,305,306,307,308或309中所示的氨基酸序列；其中所述重组核酸分子并非编码所述多肽的天然存在的序列。5.权利要求4的重组核酸分子，其中所述重组核酸分子是设计用于在植物中表达的合成序列。6.权利要求4或5的重组核酸分子，其中所述重组核酸分子与能够指导在植物细胞中的表达的启动子可操作地连接。7.权利要求4-6中任一项的重组核酸分子，其中所述重组核酸分子与能够指导在细菌中的表达的启动子可操作地连接。8.一种宿主细胞，其包含权利要求4-7中任一项的重组核酸分子。9.权利要求8的宿主细胞，其中所述宿主细胞是细菌宿主细胞。10.一种DNA构建体，其包含驱动植物细胞中的表达的启动子，所述启动子可操作地连接至重组核酸分子，所述重组核酸分子包含编码多肽的核苷酸序列，所述多肽包含与选自SEQIDNO:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85,86,87,88,89,90,91,92,93,94,95,96,97,98,99,100,101,102,103,104,105,106,107,108,109,110,111,112,113,114,115,116,117,118,119,120,121,122,123,124,125,126,127,128,129,130,131,132,133,134,135,136,137,138,139,140,141,142,143,144,145,146,147,148,149,150,151,152,153,154,155,156,157,158,159,160,161,162,163,164,165,166,167,168,169,170,171,172,173,174,175,176,177,178,179,180,181,182,183,184,185,186,187,188,189,190,191,192,193,194,195,196,197,198,199,200,201,202,203,204,205,206,207,208,209,210,211,212,213,214,215,216,217,218,218,219,220,221,222,223,224,225,226,227,228,229,230,231,232,233,234,235,236,237,238,239,240,241,242,243,244,245,246,247,248,249,250,251,252,253,254,255,256,257,258,259,260,261,262,263,264,265,266,267,268,269,270,271,272,273,274,275,276,277,278,279,280,281,282,283,284,285,286,287,288,289,290,291,292,293,294,295,296,297,298,299,300,301,302,303,304,305,306,307,308或309中所示的序列的氨基酸序列具有至少90％序列同一性百分比的氨基酸序列。11.权利要求10的DNA构建体，其中所述核苷酸序列是设计用于在植物中表达的合成DNA序列。12.一种载体，其包含权利要求10或11的DNA构建体。13.一种宿主细胞，其包含权利要求10或11的DNA构建体或者权利要求12的载体。14.一种组合物，其包含权利要求8、9或13的宿主细胞。15.权利要求14的组合物，其中所述组合物选自粉末、粉剂、丸剂、颗粒剂、喷雾剂、乳剂、胶体和溶液。16.权利要求14或15的组合物，其中所述组合物按重量计包含约1％至约99％的所述多肽。17.一种用于控制害虫群体的方法，其包括使所述害虫群体与杀虫有效量的权利要求3或14－16中任一项的组合物接触。18.一种用于产生具有杀虫活性的多肽的方法，其包括在其中编码所述多肽的核酸分子被表达的条件下，培养权利要求8、9或13中任一项的宿主细胞。19.一种具有稳定整合到其基因组内的DNA构建体的植物，所述DNA构建体包含编码具有杀虫活性的蛋白质的核苷酸序列，其中所述核苷酸序列包含：(a)编码包含SEQIDNO:1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85,86,87,88,89...

【专利技术属性】
技术研发人员：J·帕克斯，K·B·罗伯茨，R·E·塔耶尔，
申请(专利权)人：农业生物群落股份有限公司，
类型：发明
国别省市：美国,US