一种富含皮诺敛酸叶黄素酯的松子油及其制备方法技术

本发明专利技术涉及一种富含皮诺敛酸叶黄素酯的松子油及其制备方法，属于油脂加工与食品化学领域。首先，将叶黄素、溶剂加入酯化釜中，加热，搅拌保持温度0.5～3小时，降温至≤60℃，得到混合料，其次，将松子毛油加入混合料中，同时加入固定化专一性脂肪酶，保持30℃～60℃常压搅拌反应，停止加热，降至常温，反应完毕得到混合物，降温后将混合物经水洗、分层、干燥步骤去除溶剂，再经冷冻过滤去除脂肪酶与未反应的叶黄素即得到富含皮诺敛酸叶黄素酯的松子油。有益效果是：酯化脱酸降低松子油的酸价，减少油脂精炼损耗；形成皮诺敛酸叶黄素酯，给叶黄素赋予有皮诺敛酸的有效成分；反应温度低，不会对高不饱和脂肪酸及叶黄素破坏，操作简单，易于工业化生产。

Pine nut oil rich in pinocal acid lutein ester and preparation method thereof

The invention relates to a pine nut oil rich in pinenic acid lutein ester and a preparation method thereof, belonging to the field of oil processing and food chemistry. First, lutein and solvent are added to the esterification kettle, heating, stirring to keep the temperature for 0.5 to 3 hours, cooling to less than 60 degrees C, and getting the mixture. Secondly, the ppine wool oil is added to the mixture, and the immobilized specific lipase is added at the same time, and the reaction should be kept at 30 to 60 C at normal pressure, and the heating is stopped to the normal temperature, and the reaction is finished. The reaction is finished. The reaction is finished and the reaction is finished. The reaction is finished. The reaction is finished. The reaction is finished and the reaction is finished. The reaction is finished. The reaction is finished. The reaction is finished and the reaction is finished. The reaction is finished. The reaction is finished. The reaction is finished and the reaction is finished. The reaction is finished. The reaction is finished. The reaction is finished. The reaction is finished. The reaction is finished. The reaction is finished. The reaction is finished. The reaction is finished. The reaction is finished, the reaction is finished. After the mixture was obtained, the mixture was washed, stratified and dried to remove the solvent after cooling, and then the lipase and the unreacted lutein were removed by freezing and filtering to get the pine oil rich in the lutein ester rich in Pico convergent lutein. The beneficial effects are: esterification deacidification reduces acid value of pine nut oil, reduces oil refining loss, forms pinocarfate lutein ester, gives lutein an effective component with pinocarboxate, low reaction temperature, no damage to high unsaturated fatty acid and lutein, simple operation and easy to industrial production.

【技术实现步骤摘要】
一种富含皮诺敛酸叶黄素酯的松子油及其制备方法
本专利技术属于油脂加工与食品化学领域，涉及一种富含皮诺敛酸叶黄素酯的松子油及其制备方法，具体涉及松子油中的游离脂肪酸与叶黄素通过固定化酶催化酯化反应制备皮诺敛酸叶黄素酯功能油脂的方法。
技术介绍
皮诺敛酸(Pinolenicacid)为全顺式5，9，12十八碳三烯酸，它与地球上所有植物当中的不饱和脂肪酸不同，它只存在于松籽油中，不仅能够降低胆固醇(TC)、甘油三酯(TG)，升高高密度脂蛋白(HDL)，而且还能抑制、消除其他不饱和脂肪酸对机体的不利影响。同时，目前最新研究表明，它还具有抗炎、解热、镇痛，对抗各种真菌、病毒感染，促进中老年人排泄功能等作用。它被专家誉为洗血因子。成熟松子需多年生长，因此，松子脂肪中游离脂肪酸含量较高，一般松子脂肪中酸值为30-60KOHmg/g，松子油在精炼过程中，需要大量的NaOH来中和去除游离脂肪酸，且产生大量皂脚，使得产品得率偏低，造成了油脂浪费，限制了工业化生产。叶黄素酯2008年卫生部批准为新资源食品，叶黄素分子中存在共轭双键，共轭双键特别容易氧化，叶黄素的不稳定性限制了叶黄素的开发利用。叶黄素主要从本文档来自技高网...

【技术保护点】
一种富含皮诺敛酸叶黄素酯的松子油，其特征在于是由以下步骤得到的：(1)叶黄素酯化脱酸：首先，将叶黄素、溶剂加入酯化釜中，加热至30℃‑85℃，搅拌保持温度0.5～3小时，降温至≤60℃，得到混合料；其次，将松子毛油加入混合料中，同时加入固定化专一性脂肪酶，保持30℃～60℃常压搅拌反应4～24h停止加热，降至常温，反应完毕得到混合物；(2)混合物分离：降温后将混合物经水洗、分层、干燥步骤去除溶剂，再经冷冻过滤去除脂肪酶与未反应的叶黄素，即得到富含皮诺敛酸叶黄素酯的松子油。

【技术特征摘要】
1.一种富含皮诺敛酸叶黄素酯的松子油，其特征在于是由以下步骤得到的：(1)叶黄素酯化脱酸：首先，将叶黄素、溶剂加入酯化釜中，加热至30℃-85℃，搅拌保持温度0.5～3小时，降温至≤60℃，得到混合料；其次，将松子毛油加入混合料中，同时加入固定化专一性脂肪酶，保持30℃～60℃常压搅拌反应4～24h停止加热，降至常温，反应完毕得到混合物；(2)混合物分离：降温后将混合物经水洗、分层、干燥步骤去除溶剂，再经冷冻过滤去除脂肪酶与未反应的叶黄素，即得到富含皮诺敛酸叶黄素酯的松子油。2.根据权利要求1所述的一种富含皮诺敛酸叶黄素酯的松子油，其特征在于：步骤(1)所述的叶黄素要求水分≤0.1％，纯度≥80％。3.根据权利要求1所述的一种富含皮诺敛酸叶黄素酯的松子油，其特征在于：所述的溶剂包括乙醇、正己烷、丙酮一种或一种以上。4.根据权利要求1所述的一种富含皮诺敛酸叶黄素酯的松子油，其特征在于：步骤(1)中所述松子毛油需要水分≤0....

【专利技术属性】
技术研发人员：王小锋，赵尔哲，
申请(专利权)人：吉林派诺生物技术股份有限公司，
类型：发明
国别省市：吉林,22