The invention discloses a fomesfomentarius submerged fermentation culture medium and culture method to fomesfomentarius metabolic production of extracellular polysaccharide as index, on the basis of single factor test, first through the Plackett Burman design to obtain 3 main factors affecting the yield of Polysaccharide from 8 factors in: sucrose, casein peptone, inoculation amount; and then by the steepest ascent experiment to approach the optimal region; finally, according to the principle of BBD design in response surface optimization, analysis with three factors and three levels deep fermentation of polysaccharide production factors to determine the influence of fomesfomentarius extracellular. The results showed that the content of polysaccharide was 3.67g/L, which was close to the theoretical value 3.69g/L, which was 1.67 times higher than that of the optimal culture condition.
【技术实现步骤摘要】
一种木蹄层孔菌深层发酵的培养基及其培养方法
本专利技术属于生物
,涉及一种木蹄层孔菌深层发酵的培养基及其培养方法。
技术介绍
稀土和先进碳材料为国家绿色技术发展提供原料支撑,新兴市场需求巨大。我国是全球最大的粉煤灰生产国,2015年粉煤灰产量高达6.2亿吨,现阶段粉煤灰主要用于建筑水泥原料、基础工程填料、农业土壤改良等方面,经济附加值低、用途受限。通过前期分析数据表明,我国粉煤灰中稀土元素含量平均值近100μg/g,因此将粉煤灰作为稀土元素新兴来源具有重大战略意义。目前多糖的开发主要是植物原料,但是植物的过度开采会破坏生态环境,所以人工培养、微生物发酵是资源利用而且经济又环保的方法,不仅如此,微生物具有培养周期短,可工业化生产等优点。木蹄层孔菌多糖虽然具有较高的应用价值,但是木蹄层孔菌的化学成分研究相对较少。
技术实现思路
本专利技术的目的在于提供一种木蹄层孔菌深层发酵的培养基及其培养方法,旨在研究深层发酵木蹄层孔菌产多糖,为以后工业化生产多糖提供具有实际价值的参考方案。其具体技术方案为:一种木蹄层孔菌深层发酵的培养基,其配方为:蔗糖15.0g/L、酪蛋白胨7. ...
【技术保护点】
一种木蹄层孔菌深层发酵的培养基,其特征在于,其配方为:蔗糖15.0g/L、酪蛋白胨7.5g/L、接种量6.0%、KH2PO41.0g/L、MgSO40.5g/L、VB20.625mg/L、pH7.5。
【技术特征摘要】
1.一种木蹄层孔菌深层发酵的培养基,其特征在于,其配方为:蔗糖15.0g/L、酪蛋白胨7.5g/L、接种量6.0%、KH2PO41.0g/L、MgSO40.5g/L、VB20.6...
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