The invention discloses a preparation method and application of alkali dregs in fermented food. The method comprises the following steps: (1) the heat treatment milk after inoculation of Aspergillus oryzae and Aspergillus niger were cultured to obtain whey medium; (2) the whey medium hydrolysis and salt water after mixing, by hydrolysis, centrifugation, the supernatant was hydrolyzed to precipitation; (3) to hydrolysis of the supernatant by ethanol extraction, extraction liquid by refrigerated centrifuge centrifugation, supernatant, static, crystallization and removal of crystallization; (4) concentration of supernatant removal after crystallization, vacuum freeze drying, by alkali dregs. Allow the use of natural food ingredients and food industry in the microbial production of alkali dregs by the method of the invention, no fine chemical products, no two pollution, and mild reaction conditions, low energy consumption, good safety. The prepared alkali dregs can be used for the preparation of bioactive substances or flavor modifiers, or used in the preparation of compound flavor or fragrance.
【技术实现步骤摘要】
发酵食品中酒渣碱的制备方法及其应用
本专利技术涉及酒渣碱的制备
,具体涉及发酵食品中酒渣碱的制备方法及其应用。
技术介绍
酒渣碱为一种强荧光黄色的化合物,具有淡苦味,是一种吲哚生物碱衍生物。该化合物具有多种生理活性,具有抑制NF-kappaB炎症通路,具有抗HIV-1活性、抗癌活性、抗肿瘤活性、抗神经炎症活性(Dong-CheolKim,etal.Anti-neuroinflammatoryactivitiesofindolealkaloidsfromkanjang(Koreanfermentedsoysource)inlipopolysaccharide-inducedBV2microglialcells[J].Foodchemistry,2016;Bao-ning,Su.BioactiveconstituentsoftheseedsofBruceajavanica[J].PlantMed,2002.)。酒渣碱1936年第一次从日本米酒中发现,后来多次被科学家从米醋、酱油、高等植物和真菌中分离得到,并证明该化合物具有抗HIV-1活性,抗肿瘤活性。姚磊等人(姚磊,徐良雄,薛琛花,等.枝顶孢属真菌的抑菌活性及其代谢产物研究[J]热带亚热带植物学报2012,20(2):192-196.)发现酒渣碱对金黄色葡萄球菌具有较强的抑制作用;赵雪娇等(赵雪娇,王海峰,赵丹奇,等.当归化学成分的分离与鉴定[J].沈阳药科大学学报.2013,30(3):182-185.)从当归中分离纯化出酒渣碱;王洪平等(王洪平,曹芳,杨秀伟,等头花蓼地上部分的化学成分研究[J].中 ...
【技术保护点】
发酵食品中酒渣碱的制备方法,其特征在于,包括如下步骤:(1)将大豆乳清与水混合,加热处理后接种米曲霉或黑曲霉进行培养,得到乳清培养液;(2)将得到的乳清培养液与食盐水混合后进行水解,得到水解液,离心,去沉淀,得到水解上清液;(3)用无水乙醇对水解上清液进行萃取,冷冻离心,得到上清液,静置,析出结晶并去除结晶 ;(4)对去除结晶后的上清液进行浓缩,真空冷冻干燥,得到所述酒渣碱。
【技术特征摘要】
1.发酵食品中酒渣碱的制备方法,其特征在于,包括如下步骤:(1)将大豆乳清与水混合,加热处理后接种米曲霉或黑曲霉进行培养,得到乳清培养液;(2)将得到的乳清培养液与食盐水混合后进行水解,得到水解液,离心,去沉淀,得到水解上清液;(3)用无水乙醇对水解上清液进行萃取,冷冻离心,得到上清液,静置,析出结晶并去除结晶;(4)对去除结晶后的上清液进行浓缩,真空冷冻干燥,得到所述酒渣碱。2.根据权利要求1所述的发酵食品中酒渣碱的制备方法,其特征在于,步骤(1)中,所述大豆乳清与水的质量比为1:2~3;所述加热处理是在100℃下加热10~15min。3.根据权利要求1所述的发酵食品中酒渣碱的制备方法,其特征在于,步骤(1)中,所述培养是在28~32℃下摇床培养40~48h;所述米曲霉或黑曲霉的接种量为大豆乳清重量的0.02%~0.06%。4.根据权利要求1所述的发酵食品中酒渣碱的制备方法,其特征在于,步骤(2)中,所述食盐水的质量浓度为10~25%;所述乳清培养液...
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