The present invention discloses epitope polypeptide blocking chicken PD_1/PD_L1 signaling pathway and its application. The amino acid sequence of the polypeptide is VSGAEELFTVEVPQLYVV. The invention discloses an epitope polypeptide which can effectively block the PD_1/PD_L1 signaling pathway, and studies the recovery and reversal of immune suppression after blocking the PD_1/PD_L1 signaling pathway in chicken immunosuppressive state. By studying the binding ability of polypeptide to IBDV-infected chicken PBMCs, the recovery of T lymphocyte proliferation inhibition after the action of polypeptide and the gene expression of immune regulatory cytokines IL_2, IL_6 and IFN_gamma, the immunosuppression caused by activation of PD_1/PD_L1 signaling pathway becomes a potential target for clinical application of immunotherapy and serves as a polypeptide. As a new blocking inhibitor of immune checkpoint, it provides theoretical basis for obtaining polypeptide drugs and immune adjuvant vaccines to improve immune effect.
【技术实现步骤摘要】
阻断鸡PD-1/PD-L1信号通路的表位多肽及其应用
本专利技术涉及阻断鸡PD-1/PD-L1信号通路的表位多肽及其应用，属于分子病理学与免疫学
技术介绍
程序性死亡因子(programmeddeath-1，PD-1或CD279)是T细胞表面一种抑制性受体，作为免疫应答的负调节物在中枢和外周免疫反应中发挥着关键作用。PD-1有两个配体PD-Ls(LigandofProgrammed-1)分别为PD-L1和PD-L2，相比较于PD-L1，PD-L2表达更受限制，但PD-L2对PD-1具有更高的亲和力。已经证明，表达PD-1的T细胞接触表达其配体PD-L1或PD-L2的细胞时，PD-1/PD-Ls信号通路激活，抑制响应抗原刺激的T细胞受体的激活信号功能，包括增殖，细胞因子分泌和细胞毒性降低。PD-1/PD-L1是重要的免疫负调节信号通路，作为T细胞免疫反应的协同刺激信号，其在机体适应性免疫中发挥着重要的负调节作用。PD-1及其配体相互作用在感染或肿瘤消退期间或在自身耐受性发展期间下调免疫应答。慢性抗原刺激，例如在肿瘤疾病或慢性感染期间发生的刺激，导致T细胞表...
【技术保护点】
1.阻断鸡PD‑1/PD‑L1信号通路的表位多肽，其特征在于，所述多肽的氨基酸序列为：VSGAEELFTVEVPQQLYVV。
【技术特征摘要】
1.阻断鸡PD-1/PD-L1信号通路的表位多肽，其特征在于，所述多肽的氨基酸序列为：VSGAEELFTVEVPQQLYVV。2.根据权利要求1所述的阻断鸡PD-1/PD-L1信号通路的表位多肽，其特征在于，所述多肽位于PD-L2蛋白的IgV结构域A区段。3.一种如权利要求1所述的阻断鸡PD-1/PD-L1信号通路的表位多肽在IBDV感染PBMCs细胞...
【专利技术属性】
技术研发人员：王选年，孙国鹏，徐若楠，岳锋，张艳芳，李鹏，朱艳平，郭东光，何海汛，
申请(专利权)人：新乡学院，
类型：发明
国别省市：河南,41
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