The invention provides a containing methyl gallate the effective components of Radix Paeoniae Rubra, medicinal materials by heating extraction, concentration, reversed-phase silica gel column separation, elution, elution concentration and drying, and then prepared to separate liquid chromatography collection solution, solution was concentrated and dried to obtain the effective component, the main effective components containing methyl gallate. Five glucogallin. The effective component of the red peony root can be used in the preparation of medicaments for curing and preventing cardiovascular diseases. The method of the invention is reasonable in design and can obtain the effective component quickly and accurately, and is more convenient for the quality control of drugs in production.
【技术实现步骤摘要】
【技术保护点】
１．一种赤芍有效组分，其特征在于， 通过以下步骤获得：取赤芍药材，将其粉碎后加入体积比为１：１的乙酸乙酯和乙醇，加热回流１小时，提取２次，滤液合并得提取液Ｉ；药渣加入７０％乙醇溶液，加热回流１小时，提取２次，滤液合并得提取液ＩＩ，将提取液ＩＩ浓缩成浸膏，用５０％甲醇溶液溶解浸膏，采用ＯＤＳ－Ｃ１８柱对其进行分离，首先用５％甲醇溶液作为流动相，得洗脱液Ｉ，然后改用６０％甲醇溶液作为流动相，得洗脱液ＩＩ，浓缩干燥后得样品；用制备液相色谱继续分离得到的样品：制备色谱的分离条件：色谱柱为半制备柱，Ｌｉｃｈｒｏｓｐｈｅｒ Ｃ１８；１０．０ｍｍ ２５０ｍｍ，５，流动相为水Ａ和乙腈Ｂ，梯度洗脱程序如下：０ｍｉｎ， １０％Ｂ；１５ｍｉｎ， ２５％Ｂ；３４ｍｉｎ， ３０％Ｂ；流速为３ｍｌ／ｍｉｎ，柱温为室温；样品经制备液相色谱分离，在２６．８～３１．０ｍｉｎ时间段收集溶液，溶液经浓缩干燥后得到有效组分；该有效组分中主要含有没食子酸甲酯、五没食子酰葡萄糖。
【技术特征摘要】
【专利技术属性】
技术研发人员：程翼宇，王毅，范骁辉，李云飞，
申请(专利权)人：浙江大学，
类型：发明
国别省市：86
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