一种提高可卡因检测灵敏度的方法技术

本发明专利技术涉及一种提高可卡因检测灵敏度的方法，其特征在于用于可卡因检测的核酸适配体被断裂成三段，所述的方法包括如下步骤：（１）将可卡因与三段核酸适配体探针混合，使被测定的可卡因与适配体探针形成复合物；（２）进行荧光检测、电化学检测或颜色检测。由于三段核酸探针的长度比一段或两段的核酸探针显著减小，探针间，以及探针与检测体系中其它组分能够造成背景噪音的相互作用大为减少。从而显著提高可卡因的检测灵敏度。以可卡因的纳米金颜色检测为例，通过使用三段核酸探针将可卡因颜色检测的灵敏度提高了一个数量级。该方法可以推广到包括小分子和重金属离子在内的各种不同种类的物质的检测。

Method for improving sensitivity of detection of cocaine

The invention relates to a method for improving the detection sensitivity of cocaine, which is characterized in that the nucleic acid aptamer for cocaine detection is broken into three sections, wherein the method comprises the following steps: (1) and three of cocaine aptamer probes to be mixed, the determination of cocaine form complexes with aptamer probe; (2) fluorescence detection, electrochemical detection and color detection. Since the length of the three nucleic acid probe is significantly lower than that of a one or two segment nucleic acid probe, the interaction between the probe and the other components of the probe and the detection system can cause background noise to be greatly reduced. Thus, the detection sensitivity of cocaine was improved significantly. Taking the detection of gold nanoparticles in cocaine as an example, the sensitivity of cocaine color detection was increased by an order of magnitude by using three nucleic acid probes. The method can be extended to the detection of various kinds of substances, including small molecules and heavy metal ions.

【技术实现步骤摘要】

【技术保护点】
一种提高可卡因检测灵敏度的方法，其特征在于用于可卡因检测的核酸适配体被断裂成三段，所述的方法包括如下步骤：（１）将可卡因与三段核酸适配体探针混合，使被测定的可卡因与适配体探针形成复合物；（２）进行荧光检测、电化学检测或颜色检测；所述的适配体为与靶标分子结合的ＤＮＡ或ＲＮＡ分子；靶标分子为蛋白质、ＤＮＡ、小分子或金属离子。

【技术特征摘要】

【专利技术属性】
技术研发人员：娄新徽，袁敏，赵建龙，
申请(专利权)人：中国科学院上海微系统与信息技术研究所，
类型：发明
国别省市：31[中国|上海]