The invention belongs to the field of Cordyceps preparation technology, and relates to a method for improving cordycepin production by liquid fermentation of Cordyceps militaris. A method of raising Cordyceps Cordyceps liquid to produce Cordyceps, and the following steps are as follows: the potato is cooked and filtered to extract the juice, and then the glucose 10 15g, sucrose 15, 1.0g, potassium sulfate 1, 2G, peptone 2 3G, yeast cream 0.5 1g, agar 18 20g, cocoon pupae juice 200 300ml, and water 800 1000mL 1000mL 1000mL and horse bell The sweet potato juice was mixed evenly into the activated medium of bacteria, and the culture medium of mother species was prepared; adenine and adenosine were added to prepare the medium for liquid fermentation; the culture medium of Cordyceps militaris was aseptic into the culture medium, 1 1.5D was placed at the temperature of 35 degrees C, and cultured in an oscillating incubator, and the liquid bacteria were inoculated after sterilization. In the mother culture medium, 3 5D was cultivated and then cultured in the medium of liquid fermentation, and then cultured in the medium of 8. The product was obtained. The preparation method was mixed with adenine and adenosine, and the production of cordycepin could be improved by adding liquid medium, and the operation was simple.
【技术实现步骤摘要】
一种提高蛹虫草液体生产虫草菌素的方法
本专利技术属于虫草制备
,涉及一种提高蛹虫草液体生产虫草菌素的方法。
技术介绍
虫草素具有多种生物活性,有抗菌、降血脂和抗癌等作用。目前蛹虫草是主要产虫草素的菌株,蛹虫草的液体发酵研究主要集中在发酵条件的优化及有效成分的检测方面。而对于发酵过程中有效成分的动态积累研究甚少,工业生产中,既要获得目标产物的最大量,还要节省生产成本并且存在子实体生长周期长,虫草素的含量低,提取虫草素成本高等缺点,因此,研制一种提高蛹虫草液体生产虫草菌素的方法是非常必要的。
技术实现思路
针对现有技术存在的问题,本专利技术的目的在于提供一种提高蛹虫草液体生产虫草菌素的方法。本专利技术制备方法采用添加腺嘌呤和腺苷的混合添加物,加入液体培养基能提高虫草素产量并且操作简单。为解决上述技术问题,本专利技术采用的技术方案为。一种提高蛹虫草液体生产虫草菌素的方法,步骤如下:(1)将马铃薯150-200g和1000-1500ml水放入容器中加热煮熟,过滤取汁500-700ml,再将葡萄糖10-15g,蔗糖15-18g,硫酸镁0.5-1.0g,硫酸钾1-2g,蛋白胨2-3g,酵母膏0.5-1g,琼脂18-20g,茧蛹汁200-300ml,和水800-1000mL与马铃薯汁混合均匀配制成菌种活化培养基;(2)秤取葡萄糖5-15g,蔗糖10-15g,蛋白胨1-3g,酵母膏1-2g,硫酸镁0.2-1.0g,硫酸钾0.5-1.0g和水800-1000mL放入容器中混合均匀,将其在温度为120-125℃下灭菌25-35min配制得母种培养基;(3)秤取葡萄糖5-15 ...
【技术保护点】
一种提高蛹虫草液体生产虫草菌素的方法,其特征在于,步骤如下:(1)将马铃薯150‑200g和1000‑1500ml水放入容器中加热煮熟,过滤取汁500‑700ml,再将葡萄糖10‑15g,蔗糖15‑18g,硫酸镁 0.5‑1.0g,硫酸钾1‑2g,蛋白胨2‑3g,酵母膏0.5‑1g,琼脂18‑20g,茧蛹汁200‑300ml,和水800‑1000mL 与马铃薯汁混合均匀配制成菌种活化培养基;(2)秤取葡萄糖5‑15g,蔗糖10‑15g,蛋白胨1‑3g,酵母膏1‑2g,硫酸镁 0.2‑1.0g,硫酸钾0.5‑1.0g和水800‑1000mL放入容器中混合均匀, 将其在温度为120‑125℃下灭菌25‑35min配制得母种培养基;(3)秤取葡萄糖5‑15g,红糖10‑15g,蛋白胨1‑3g,酵母膏1‑2g,硫酸镁 0.2‑1.0g,硫酸钾0.5‑1.0g和水800‑1000mL放入容器中混合均匀,以0 .25um微滤膜除去杂质后加入腺嘌呤和腺苷,调至质量浓度0.9‑1.2g/L,将其在温度为120‑125℃下灭菌25‑35min配制得液体发酵基础培养基;(4)在菌种活化培养基中按无菌操作接 ...
【技术特征摘要】
1.一种提高蛹虫草液体生产虫草菌素的方法,其特征在于,步骤如下:(1)将马铃薯150-200g和1000-1500ml水放入容器中加热煮熟,过滤取汁500-700ml,再将葡萄糖10-15g,蔗糖15-18g,硫酸镁0.5-1.0g,硫酸钾1-2g,蛋白胨2-3g,酵母膏0.5-1g,琼脂18-20g,茧蛹汁200-300ml,和水800-1000mL与马铃薯汁混合均匀配制成菌种活化培养基;(2)秤取葡萄糖5-15g,蔗糖10-15g,蛋白胨1-3g,酵母膏1-2g,硫酸镁0.2-1.0g,硫酸钾0.5-1.0g和水800-1000mL放入容器中混合均匀,将其在温度为120-125℃下灭菌25-35min配制得母种培养基;(3)秤取葡萄糖5-15g,红糖10-15g,蛋白胨1-3g,...
【专利技术属性】
技术研发人员:王弘,
申请(专利权)人:辽宁弘侨生物科技股份有限公司,
类型:发明
国别省市:辽宁,21
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