The invention discloses a method for preparing a tumor targeting nanoparticle carrier of MTDH siRNA, which is a chemotherapeutic drug of breast cancer, which is used to dissolve polyethyleneimine glycolic acid polymer in dichloromethane and add deionized water. Then the above solution is broken into a homogeneous emulsion after ultrasonic fragmentation, and the ethylene alcohol and hydrophobicity are made. After the mixture of paclitaxel and dichloromethane, the emulsion was added to the emulsion, then the emulsion was emulsified after ultrasonic breakage. The emulsion was evaporated to get the suspension of the nanoparticles. Then the nanoparticle suspension containing paclitaxel was prepared and then washed, stirred and centrifuged. The invention can carry the breast cancer chemotherapeutic drugs and nucleic acids into the breast cancer cells with high expression of MTDH gene simultaneously, inhibit cell proliferation, and have significant tumor targeting in vitro and in vivo. The antitumor effect of the invention is clear, and the carrier material has high biological safety, good biocompatibility, biodegradability, innocuity, and no immunogenicity. The preparation process is simple, easy to operate, save time and energy, and is suitable for large-scale production.
【技术实现步骤摘要】
共载有乳腺癌化疗药物MTDHsiRNA的肿瘤靶向纳米粒子载体的制备方法
本专利技术属于纳米药物领域,具体地涉及共载乳腺癌化疗药物MTDHsiRNA的肿瘤靶向纳米粒子的制备方法。技术背景恶性肿瘤是威胁人类健康与社会发展的重要疾病,其发生是多基因、多因素相互作用的复杂过程,最终导致细胞异常增殖与死亡的结果。乳腺癌已成为女性发病率最高的肿瘤,并且乳腺癌作为一种高度异质性肿瘤,在病理分型、分子分型及预后等方面存在显著差异。雌激素受体阳性乳腺癌虽可从内分泌治疗中获益,但化疗在术后系统治疗和复发转移后的治疗中仍占重要地位。而三阴性乳腺癌因其侵袭性强、术后易复发转移且不能从内分泌治疗及抗HER-2靶向治疗中获益,故化疗成为唯一的全身治疗手段。但随着治疗时间的延长,终出现药物耐药和复发转移。MTDH基因,即转移黏附基因,又称星形细胞上调基因-1(astrocyteelevatedgene-1,AEG-1)和LYRIC,是一个发现仅十余年的原癌基因。现有研究已证实MTDH基因在诸如肝癌、胃癌、肺癌、结肠癌、卵巢癌、子宫内膜癌、乳腺癌、前列腺癌、恶性胶质瘤等多种恶性肿瘤中高表达。MTDH基因过表达在多种肿瘤的发生发展过程中起到重要的作用,并与肿瘤细胞侵袭、扩散、转移及化疗耐药相关。现有研究结果显示,MTDH过表达可导致肿瘤细胞对5-FU、阿霉素、顺铂、紫杉醇、他莫昔芬以及分子靶向药物等多种化疗药物产生耐药。相反的,当敲除MTDH基因或MTDH基因表达降低,则可以促进肿瘤细胞凋亡、增加化疗敏感性。研究显示,使用siRNA抑制MTDH表达可诱导前列腺癌细胞凋亡,并增加了细胞对 ...
【技术保护点】
共载有乳腺癌化疗药物MTDH siRNA的肿瘤靶向纳米粒子载体的制备方法,其特征在于包括以下步骤:a.将18~22mg聚乙烯亚胺‑乳酸羟基乙酸聚合物溶于1ml二氯甲烷中后,加入0.2~0.3ml去离子水混匀;b.将上述溶液超声破碎后乳化成均一乳液;c.将1.8~2.2ml质量浓度为1.8~2.2%的乙烯醇和0.2~0.3ml质量浓度为1.8~2.2%的疏水性紫杉醇与2~4ml二氯甲烷混合后,加入上述乳液中,然后在持续滴加10ml质量浓度为0.6%的乙烯醇状态下再次超声破碎后乳化成均一乳液;d.将c步骤制得的乳液蒸发去除有机相后,得到包载有紫杉醇的纳米粒子核心;e.将上述包载有紫杉醇的纳米粒子核心用去离子水在12000~13000转/分转速下漂洗5~8分钟后,在200转/分转速下搅拌20~25分钟,然后在2000~2200转/分转速下离心5~8分钟。
【技术特征摘要】
1.共载有乳腺癌化疗药物MTDHsiRNA的肿瘤靶向纳米粒子载体的制备方法,其特征在于包括以下步骤:a.将18~22mg聚乙烯亚胺-乳酸羟基乙酸聚合物溶于1ml二氯甲烷中后,加入0.2~0.3ml去离子水混匀;b.将上述溶液超声破碎后乳化成均一乳液;c.将1.8~2.2ml质量浓度为1.8~2.2%的乙烯醇和0.2~0.3ml质量浓度为1.8~2.2%的疏水性紫杉醇与2~4ml...
还没有人留言评论。发表了对其他浏览者有用的留言会获得科技券。