The present invention relates to cereal cyst nematode Ha 16674 protein, encoding gene and its application. A cereal cyst nematode Ha 16674 amino acid protein, the SEQ ID NO:1 column shown. Encoding of cereal cyst nematode Ha 16674 protein gene, the full-length cDNA nucleotide sequence of SEQ ID NO:2. The experiment shows that Ha and Bax were 16674 injection of tobacco leaves, can inhibit Bax induced leaf cell death. The full-length of Ha in Arabidopsis 16674 gene over expression, access to genetic stability of T3 transgenic positive plants. Calreticulin Ha CCN 16674 can reduce the immune defense response in Arabidopsis, promoting Pseudomonas syringae infection. The present invention for the cultivation of plant nematode resistance provides a new target for Ha 16674, has a good theoretical guidance for the development of broad-spectrum anti SCN crops.
【技术实现步骤摘要】
禾谷孢囊线虫Ha-16674蛋白、编码基因及其应用
本专利技术属于生物
,涉及一个来自禾谷孢囊线虫基因Ha-16674基因序列和编码蛋白及其应用。
技术介绍
小麦禾谷孢囊线虫(Heteroderaavenae)是危害小麦、大麦、燕麦等禾本科作物的重要病原线虫,目前已在37个国家如美国、加拿大、澳大利亚、法国、英格兰、挪威、意大利、印度、土耳其、叙利亚、伊朗等国家发生(NicolJ,I,BolatN,etal.Theglobalimportanceofthecerealcystnematode(Heteroderaspp.)onwheatandinternationalapproachestoitscontrol[J].Communicationsinagriculturalandappliedbiologicalsciences,2006,72(3):677-686)。在澳大利亚CCN危害面积高达200万公顷,一般产量损失为23-50%,严重时损失高达73-89%,年经济损失7000万美元(BrownR.Culturalpracticesandtheiref ...
【技术保护点】
一种禾谷孢囊线虫Ha‑16674蛋白,其氨基酸列SEQ ID NO:1所示。
【技术特征摘要】
1.一种禾谷孢囊线虫Ha-16674蛋白,其氨基酸列SEQIDNO:1所示。2.编码权利要求1所述的禾谷孢囊线虫Ha-16674蛋白的基因。3.根据权利要求2所述基因,其cDNA全长核苷酸序列如SEQIDNO:2。...
【专利技术属性】
技术研发人员:彭德良,刘敬,彭焕,黄文坤,孔令安,崔江宽,王高峰,乔芬,罗书介,李新,
申请(专利权)人:中国农业科学院植物保护研究所,
类型:发明
国别省市:北京,11
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