一种人骨肉瘤细胞株及其应用制造技术

本发明专利技术提供了一种人骨肉瘤细胞株及其应用。该人骨肉瘤细胞株保藏在中国典型培养物保藏中心，保藏编号为ＣＣＴＣＣ　ＮＯ：Ｃ２０１００２。该人骨肉瘤细胞株可用于在哺乳动物中产生骨肉瘤，制备骨肉瘤动物模型，可进一步用于筛选治疗骨肉瘤的候选药物。本发明专利技术的人骨肉瘤细胞株性状稳定，可稳定多次传代。体外传代自第３０代至第９０代性状保持稳定。本发明专利技术的骨肉瘤细胞株具有临床上骨肉瘤的生物学性状，尤其是同时具备原发耐药和高转移潜能的特性。对于解决原发耐药和远处转移这两个目前骨肉瘤治疗中无法回避的瓶颈问题，具有重要意义，是人骨肉瘤基础研究和临床前期应用的理想细胞株。

Human osteosarcoma cell line and application thereof

The present invention provides a human osteosarcoma cell strain and its application. The human osteosarcoma cell line is preserved in a typical Chinese culture collection center, and the preservation number is CCTCC NO:C201002. The osteosarcoma cell line can be used to produce osteosarcoma in mammals, and an animal model of osteosarcoma can be prepared, which can further be used as a candidate drug for the treatment of osteosarcoma. The human osteosarcoma cell line of the invention has stable characters and can be stably passaged several times. The characters remained stable from thirtieth to ninetieth generations. The osteosarcoma cell line of the invention has the biological characteristics of osteosarcoma in clinic, in particular, has the characteristics of primary resistance and high metastatic potential. It is of great significance for the treatment of the primary resistance and distant metastasis of the two current osteosarcoma therapies. It is an ideal cell line for basic research and clinical application of osteosarcoma.

【技术实现步骤摘要】

【技术保护点】
１．一种人骨肉瘤细胞，其特征在于，其保藏在中国典型培养物保藏中心，保藏编号为ＣＣＴＣＣ－Ｃ２０１００２。

【技术特征摘要】

【专利技术属性】
技术研发人员：姚阳，沈赞，余文熙，
申请(专利权)人：姚阳，沈赞，余文熙，
类型：发明
国别省市：31[]