The invention belongs to the technical field of bioengineering. Specifically, it involves the pig ileal epithelial cell lines which are susceptible to all three kinds of porcine enteric coronavirus. Through the cloning and screening of IPI \u2011 2I cells, a cell line IPI \u2011 FX, which is sensitive to porcine epidemic diarrhea virus (PEDV), porcine delta coronavirus (pdcov) and porcine transmissible gastroenteritis virus (TGEV), was obtained. The cell line was deposited in China typical culture preservation center, and its preservation number is CCTCC No: c2019115. PEDV, pdcov and TGEV can effectively proliferate on IPI \u2011 FX cells, and the proliferation titer is higher than 10
【技术实现步骤摘要】
对三种猪肠道冠状病毒易感的猪回肠上皮细胞株
本专利技术属于生物工程
,具体涉及对三种猪肠道冠状病毒(包括PEDV、TGEV、PDCoV)易感的猪回肠上皮细胞株。
技术介绍
冠状病毒(Coronaviruses,CoV)是不分节段的单股正链RNA病毒,分为α冠状病毒属(Alphacoronavirus,α-CoV),β冠状病毒属(Betacoronavirus,β-CoV),γ冠状病毒属(Gammacoronavirus,γ-CoV)和δ冠状病毒属(Deltacoronavirus,δ-CoV)4个属(Suetal.Epidemiology,geneticrecombination,andpathogenesisofcoronaviruses.TrendsMicrobiol.201624(6):490-502)。猪肠道冠状病毒是引起仔猪腹泻的主要病毒,迄今为止,已报道的能引起猪群大规模腹泻的猪肠道冠状病毒主要包括传染性胃肠炎病毒(transmissiblegastroenteritisvirus,TGEV),猪流行性腹泻 ...
【技术保护点】
1.一种对三种猪肠道冠状病毒易感的猪回肠上皮细胞株IPI-FX,保藏在中国典型培养物保藏中心,保藏编号为CCTCC NO:C2019115。/n
【技术特征摘要】
1.一种对三种猪肠道冠状病毒易感的猪回肠上皮细胞株IPI-FX,保藏在中国典型培养物保藏中心,保藏编号为CCTCCNO:C2019115。
2.如权利要求1所述的对三种猪肠道冠状病毒易感的猪回肠上皮细胞株IPI-FX...
【专利技术属性】
技术研发人员:方六荣,肖少波,汪训磊,刘康,王荡,方谱县,
申请(专利权)人:华中农业大学,
类型:发明
国别省市:湖北;42
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