OsNPF8.8b基因在提高水稻产量和营养品质中的应用制造技术
Application of OsNPF8.8b gene in improving rice yield and nutritional quality
The invention discloses the application of OsNPF8.8b gene in improving rice yield and nutritional quality, and belongs to the field of plant genetic engineering. The amino acids of the OsNPF8.8b gene encoding proteins and their cDNA sequences are shown in SEQ ID ID NO.1 and 2. By constructing the overexpression plant of rice OsNPF8.8b gene, it was found that increasing the expression of OsNPF8.8b gene could increase the number of rice tillers and effective spikes, increase the grain filling grain number of single plant and the dry weight of grain filling grain, and increase the globulin content in rice. By constructing the mutant plant, it was found that the number of rice tillers decreased, the number of grain filling grain and the dry weight of grain filling in single plant decreased, and the content of globulin in rice decreased with the expression of OsNPF8.8b gene. Therefore, OsNPF8.8b gene can be used to improve rice yield and quality, and it has important application in improving nitrogen use efficiency and improving rice quality.
【技术实现步骤摘要】
OsNPF8.8b基因在提高水稻产量和营养品质中的应用
本专利技术属于植物基因工程领域，具体涉及OsNPF8.8b基因在提高水稻产量和营养品质中的应用。
技术介绍
植物通过吸收土壤中的铵根、硝酸根、氨基酸、可溶性肽等来获得氮素；氮的吸收和转运主要依靠铵根运输蛋白(AMT)、硝酸根运输蛋白(NRT)、氨基酸运输蛋白(AAT)、肽运输蛋白(PTR)等运输蛋白来完成(WilliamsL,MillerA.Transportersresponsiblefortheuptakeandpartitioningofnitrogenoussolutes.AnnualReviewofPlantPhysiologyandPlantMolecularBiology,2001,52:659-688.)。铵通过植物AMT吸收后再通过谷氨酰胺合成酶(GS)和谷氨酸合酶(GOGAT)合成谷氨酰胺和谷氨酸，后者再进一步形成其它氨基酸(SonodaY,IkedaA,SaikiS,etal.FeedbackregulationoftheammoniumtransportergenefamilyAMT1...
【技术保护点】
OsNPF8.8b基因在提高水稻分蘖数中的应用。
【技术特征摘要】
1.OsNPF8.8b基因在提高水稻分蘖数中的应用。2.OsNPF8.8b基因在提高水稻有效穗数中的应用。3.OsNPF8.8b基因在提高水稻产量中的应用。4.根据权利要3所述的应用，其特征在于：所述的水稻产量包括水稻单株籽粒粒数和单株籽粒干重。5.OsNPF8.8b基因在改良水稻营养品质中的应用。6.根据权利要求5所述的应用，其特征在于：所述改良水稻营养品质包括增加大米中球蛋白含量。7.根据权利要求1-6任一项所述的应用，其特征在...
【专利技术属性】
技术研发人员：方中明，聂海鹏，黄玮婷，吕凯，汪杰，
申请(专利权)人：武汉生物工程学院，
类型：发明
国别省市：湖北,42
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