A method of renaturation and purification of Recombinant Human Granulocyte Colony-stimulating Factor is made by removing reducing agent by replacement of buffer solution before refolding, which improves the controllability and refolding rate of the refolding process and optimizes the composition of the complex buffer solution. The above method is suitable for large-scale industrial production, low demand for equipment, simple operation, high purity of protein and good stability. It can reduce the side effects of the final product in clinical, and improve the safety and effectiveness of the drug.
【技术实现步骤摘要】
【国外来华专利技术】PCT国内申请，说明书已公开。
【技术保护点】
PCT国内申请，权利要求书已公开。
【技术特征摘要】
【国外来华专利技术】2016.04....
【专利技术属性】
技术研发人员：张晨光，王宏伟，徐峰，汪军，齐艳艳，
申请(专利权)人：江苏恒瑞医药股份有限公司，
类型：发明
国别省市：江苏,32
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