The invention provides a preparation method of quaternary ammonium salt type antibacterial coating which can stimulate the responsive release. The coating has good sterilization effect while achieving the responsive release of therapeutic drugs. Preparation method of the coating material is prepared with redox responsive polymer two dimethylamino ethyl bromide twelve poly 1 alkyl methacrylate quaternary ammonium salt Co N [2 by free radical polymerization (pyridine 2 two alkyl sulfur) ethyl] acrylamide methyl methacrylate (CO PPDM), further polymer and small molecule drugs dissolved in an organic solvent and solvent were prepared by selective redox response of drug loaded colloidal particles, the above-mentioned drug loaded colloidal particle dispersions as electrolyte by electrophoretic deposition technique on 316L stainless steel surface coating. The coating not only has good inhibitory effect on Escherichia coli and Staphylococcus aureus, but also through the response of the reducing substances cases of glutathione achieve controlled release of drug; at the same time, the coating preparation method has the advantages of simple process, controllable conditions, suitable substrate, wide drug loading rate is high.
【技术实现步骤摘要】
一种同时具有响应性药物释放和抗菌功能的涂层及其制备方法
本专利技术涉及一种同时具有响应性药物释放和抗菌功能的涂层及其制备方法,属于抗菌表面、抗菌涂层领域。技术背景生物医用金属材料临床应用时易引起细菌粘附、增殖,进一步造成细菌感染,引发炎症、疼痛、发热等一系列并发症。在金属材料表面制备抗菌涂层成为改善上述问题的主要方法,目前,抗菌涂层主要分为两类:接触型和缓释型。接触型抗菌涂层主要通过在涂层中引入季铵盐,其可破坏细菌细胞膜,造成细菌细胞内蛋白质和营养物质流失,导致细菌死亡;(ShadyFarah,OrenAviv,NataliaLaout,etal.Quaternaryammoniumpoly(diethylaminoethylmethacrylate)possessingantimicrobialactivity.ColloidsandSurfacesB:Biointerfaces,2015,128:608-613.)缓释型抗菌涂层主要是通过负载抗菌剂或金属粒子,通过其释放杀死细菌(ChangliZhao,PengHou,JiahuaNi,etal.Ag-IncorporatedFHACoatingonPureMg:DegradationandinVitroAntibacterialProperties.ACSAppl.Mater.Interfaces2016,8:5093-5103.)。但是缓释型抗菌涂层中药物释放不可控,且长期使用易使细菌产生抗药性;同时,患者伤口部位细菌感染后,会出现炎症、发热、疼痛等并发症,需要施加一定量药物进行治疗。因此,制备 ...
【技术保护点】
一种同时具有响应性药物释放和抗菌双重功能的涂层及其制备方法,如附图1。
【技术特征摘要】
1.一种同时具有响应性药物释放和抗菌双重功能的涂层及其制备方法,如附图1。2.权利要求1中所述涂层制备方法依次包括下列步骤:(1)氧化还原响应性无规共聚物PPDM的制备:分别称取N-[2-(吡啶-2-二硫烷基)-乙基]-丙烯酰胺PDSA、1-溴十二烷基甲基丙烯酸二甲氨基乙酯季铵盐DMAC12、甲基丙烯酸甲酯MMA及引发剂2,2-偶氮二异丁氰AIBN溶于有机溶剂中,室温下搅拌溶解,在氮气氛围中反应。反应完成后将反应液在无水乙醚中反复沉淀三次,真空干燥即可得到无规共聚物;三种单体PDSA、DMAC12、MMA的投料摩尔比范围为6:4:0-5:3:2,引发剂加入量占单体总摩尔数的1%-2%,反应温度为60℃-80℃,反应时间为12-24h。(2)载药胶体粒子的制备:将步骤(1)中所制备得到的无规共聚物PPDM和药物分子共同溶解于有机溶剂中,接着向溶液中...
【专利技术属性】
技术研发人员:刘晓亚,李杨,朱叶,孟龙,魏玮,
申请(专利权)人:江南大学,
类型:发明
国别省市:江苏,32
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