The invention relates to a steam fog type production method of mycorrhizal fungi and production equipment, including equipment and spray nozzle, spray chamber controller, nutrition supply device, nutrition supply pipe spray nozzle in the growth chamber on the supply pipe nutrient solution in the growth chamber bottom perforation into the submersible pump nutrition supply in the box below the bottom of the growth and growth at the bottom of the box are connected; the reflux hole of nutrient solution, connected through the return pipe and nutrient solution box tube. The host plants of inoculated hyphae were placed in the air, and the root spray nutrient solution was sprayed every few seconds with the nozzle at the bottom of the roots. Mycorrhizal fungi grew at the root of the plant and produced spores. Due to the improvement of ventilation, the host plant roots and hyphae to avoid the pollution of soil microorganism to the root system, easy to obtain pure fungal propagules per gram, the final mixture of dry weight root produced with 6000 spores averagely, suitable for large-scale industrialized production.
【技术实现步骤摘要】
【技术保护点】
一种汽雾式菌根真菌的生产方法，其特征在于包括如下步骤： （１）、宿主植物的选择 选取的宿主植物为白喜草（Ｐａｓｐａｌｕｍ ｎｏｔａｔｕｍ Ｆｌｕｇｇｅ）； （２）、菌根真菌的菌种选用 选用包括Ｇｌｏｍｕｓ ｉｎｔｒａｒａｄｉｃｅｓ，Ｇｌｏｍｕｓ ａｇｇｒｅｇａｔｕｍ，Ｇｌｏｍｕｓｍｏｓｓｅａｅ三种混合丛枝菌根真菌的繁殖体的菌剂，每克菌根真菌的混合物有１５０个孢子； （３）、白喜草与菌根真菌共生生长时用的营养液，即气雾营养液的配方为： 母液： 每升营养液所用的量（ｍ０ｃｍ长，其余的部分用剪刀剪下，将根与菌根真菌一起晒干后切成０．５ｃｍ的碎段，每克干重根所产生的混合物含孢子数平均为６０００个，与矽藻土按重量比为１∶４０的比例混合后装袋，混合后的孢子数为每克１５０个； ④、收获后菌根真菌的保藏 与矽藻土混合好的菌根真菌样品存放在４℃的冷藏库内备用。Ｌ／Ｌ） ０．０１Ｍ ＫＨ↓［２］ＰＯ↓［４］ ０．３ １．００Ｍ ＫＮＯ↓［３］ １．５ １．００Ｍ Ｃａ（ＮＯ↓［３］）↓［２］．４Ｈ↓［２］Ｏ １．５ １．００Ｍ ＭｇＳＯ↓［４］．７Ｈ↓［２］Ｏ ０．３ ０．１Ｍ ...
【技术特征摘要】
【专利技术属性】
技术研发人员：郎咸白，
申请(专利权)人：上海卉芸农业科技有限公司，
类型：发明
国别省市：31[中国|上海]
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