检测膀胱及泌尿道上皮癌的尿液小RNA指纹图谱及其应用制造技术

本发明专利技术涉及miRNA指纹图谱在人膀胱及泌尿道上皮癌(包括膀胱癌、肾盂癌、输尿管癌、排尿管癌等)诊断和治疗中的应用，由miRNA组成的多个指纹图谱能够非常有效地区分膀胱及泌尿道上皮癌的尿液样本与非膀胱及泌尿道上皮癌的尿液样本，灵敏度高，特异性强。可有效地用于膀胱及泌尿道上皮癌的检测，早期诊断与筛查，及膀胱及泌尿道上皮癌药物的筛选。膀胱及泌尿道上皮癌药物的筛选。

【技术实现步骤摘要】
1260,hsa-miR-133a,hsa-miR-429，hsa-miR-143#, hsa-miR-29c#,hsa-miR-29c,hsa-miR-152,hsa-miR-100,hsa-miR-27b，和 hsa-miR-497。
[0008]在另一方面，本申请提供了一种诊断待测个体是否患有膀胱及泌尿道上皮癌或者是否处于患膀胱及泌尿道上皮癌高风险的方法，包括：
[0009]a)获得所述待测个体的待测尿液样本；
[0010]b)测定所述待测尿液样本中本申请提供的miRNA组合中的每个miRNA的表达水平；和
[0011]c)通过所述miRNA的表达水平评估所述待测个体是否患有膀胱及泌尿道上皮癌或者是否处于患膀胱及泌尿道上皮癌的高风险。
[0012]在某些实施方式中，所述miRNA组合包括至少3个、至少4个、至少5个、至少6个、至少7个、至少8个、至少9个，至少10个，至少11个，至少12 个，至少13个，至少14个，至少15个，或至少16个选自下组的miRNA： hsa-miR-99a,hsa-miR-141,hsa-miR-151-5p,本文档来自技高网...

【技术保护点】

【技术特征摘要】
429，hsa-miR-143#,hsa-miR-29c#，和hsa-miR-27b；或者包括：hsa-miR-100,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-429，hsa-miR-143#,hsa-miR-29c#，和hsa-miR-27b；5)所述组合5包括：hsa-miR-99a,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-429，hsa-miR-143#,hsa-miR-29c#,和hsa-miR-152；或者包括：hsa-miR-100,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-429，hsa-miR-143#,hsa-miR-29c#,和hsa-miR-152；6)所述组合6包括：hsa-miR-99a,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-183,hsa-miR-1260,hsa-miR-133a,hsa-miR-143#,hsa-miR-29c,和hsa-miR-27b；或者包括：hsa-miR-100,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-183,hsa-miR-1260,hsa-miR-133a,hsa-miR-143#,hsa-miR-29c,和hsa-miR-27b；7)所述组合7包括：hsa-miR-99a,hsa-miR-141,hsa-miR-151-5p,hsa-miR-1260,hsa-miR-429，hsa-miR-143#,hsa-miR-29c#,hsa-miR-29c,和hsa-miR-152；或者包括：hsa-miR-100,hsa-miR-141,hsa-miR-151-5p,hsa-miR-1260,hsa-miR-429，hsa-miR-143#,hsa-miR-29c#,hsa-miR-29c,和hsa-miR-152；8)所述组合8包括：hsa-miR-99a,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-1260,hsa-miR-133a,hsa-miR-429，hsa-miR-143#,hsa-miR-29c#,hsa-miR-29c,和hsa-miR-152；或者包括：hsa-miR-100,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-1260,hsa-miR-133a,hsa-miR-429，hsa-miR-143#,hsa-miR-29c#,hsa-miR-29c,和hsa-miR-152；9)所述组合9包括：hsa-miR-99a,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-1260,hsa-miR-143#,hsa-miR-29c#,hsa-miR-29c,hsa-miR-152,和hsa-miR-100；10)所述组合10包括：hsa-miR-99a,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-1260,hsa-miR-133a,hsa-miR-143#,hsa-miR-29c#,hsa-miR-29c,hsa-miR-152，和hsa-miR-27b，或者包括hsa-miR-100,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-1260,hsa-miR-133a,hsa-miR-143#,hsa-miR-29c#,hsa-miR-29c,hsa-miR-152，和hsa-miR-27b；11)所述组合11包括：hsa-miR-99a,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-1260，hsa-miR-133a,和hsa-miR-429，或者包括：hsa-miR-100,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-1260，hsa-miR-133a,和hsa-miR-429；12)所述组合12包括：hsa-miR-99a,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183，和hsa-miR-133a，或者包括：hsa-miR-100,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183，和hsa-miR-133a；13)所述组合13包括：hsa-miR-99a,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-1260,hsa-miR-133a,hsa-miR-429,和hsa-miR-29c，或者包括hsa-miR-100,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-1260,hsa-miR-133a,hsa-miR-429,和hsa-miR-29c；14)所述组合14包括：hsa-miR-99a,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-1260，hsa-miR-133a,hsa-miR-429,和hsa-miR-497，或者
包括：hsa-miR-100,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-1260，hsa-miR-133a,hsa-miR-429,和hsa-miR-497；15)所述组合15包括：hsa-miR-99a,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-1260,hsa-miR-133a,hsa-miR-429，hsa-miR-143#,和hsa-miR-29c，或者包括：hsa-miR-100,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-1260,hsa-miR-133a,hsa-miR-429，hsa-miR-143#,和hsa-miR-29c；16)所述组合16包括：hsa-miR-99a,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-133a,hsa-miR-143#,和hsa-miR-29c#，或者hsa-miR-100,hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-133a,hsa-miR-143#,和hsa-miR-29c#；17)所述组合17包括：hsa-miR-99a，hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-133a,hsa-miR-429，hsa-miR-143#,hsa-miR-29c#，或者包括hsa-miR-100，hsa-miR-141,hsa-miR-151-5p,hsa-miR-96,hsa-miR-125b,hsa-miR-183,hsa-miR-133a,hsa-miR-429，hsa-miR-143#,hsa-miR-29c#；18)所述组合18包括：hsa-miR-100,hsa-miR-125b,hsa-miR-133a,hsa-miR-141,hsa-miR-143#,hsa-miR-151-5p,hsa-miR-29c,hsa-miR-29c#,hsa-miR-99a,和hsa-miR-96；和19)所述组合19包括：hsa-miR-100,hsa-miR-1...

【专利技术属性】
技术研发人员：谭若颖，吴鸿菲，张奉武，王海龙，
申请(专利权)人：上海翔琼生物技术有限公司，
类型：发明
国别省市：