The invention relates to a new component of L lactic acid fermentation, in particular to the production technology of L lactic acid by multi-strain fermentation, which is applied to the fermentation process of L lactic acid and belongs to the biochemical technology. Different strains grow at different rates in a certain sugar solution. To obtain high concentration of fermentation broth, the fermentation period is more than 40 hours. To reduce the fermentation period, the concentration of fermentation broth is not high, generally below 150 G/L. By means of multi-strains joining fermentation at the same time, when the initial sugar concentration is high, the strain adapted to high sugar will grow and metabolize, and the low sugar-tolerant strain will regain its vitality after the sugar concentration is reduced. The invention has the advantages of keeping the fermentation acid-producing speed unchanged. Increase the concentration of fermentation broth, reduce the energy consumption of evaporation in the back channel, shorten the fermentation cycle and improve the utilization rate of equipment.
【技术实现步骤摘要】
L-乳酸发酵新组份所属
本专利技术是一种L-乳酸发酵新组份,特别是多菌种发酵制作L-乳酸的生产技术,应用于L-乳酸发酵过程,属于生物化工技术。
技术介绍
目前,L-乳酸发酵大多采用先配制一定浓度的糖溶液,经灭菌后接种发酵,再用添加钙盐来控制发酵的PH,及时移去菌种代谢的产物,使发酵液PH控制在有利于菌种生长范围内。不同的菌种在一定糖溶液中的生长速度不一样,要得到高浓度的发酵液,发酵周期就超过40小时;要降少发酵周期,发酵液浓度就不高,一般在150G/L以下。这样导致发酵能量消耗大,设备利用率低,产品成本高。为此设计出一种两全其美的多菌种发酵的制作方法是生物化工企业所面临的研发课题。
技术实现思路
为了克服现有工艺方法的缺陷,本专利技术提供一种L-乳酸发酵新组份,提高了浓度的同时,缩短了发酵周期。本专利技术解决解决其技术问题的方案是:选用专用凝结芽孢杆菌、嗜热乳酸杆菌、鼠李糖乳杆菌混合加入,组份及百分重量为:葡萄糖5%、牛肉膏2%、酵母膏0.5%、蛋白胨0.5%、磷酸二氢钾0.05%、硫酸镁0.05%、氧化钠0.02%、硫酸锰0.01%、氢氧化钙1%、琼脂1.5%。选育 ...
【技术保护点】
1.一种L‑乳酸发酵新组份,其特征在于:选用专用凝结芽孢杆菌、嗜热乳酸杆菌、鼠李糖乳杆菌混合加入,组份及百分重量为:葡萄糖5%、牛肉膏2%、酵母膏0.5%、蛋白胨0.5%、磷酸二氢钾0.05%、硫酸镁0.05%、氧化钠0.02%、硫酸锰0.01%、氢氧化钙1%、琼脂1.5%。
【技术特征摘要】
1.一种L-乳酸发酵新组份,其特征在于:选用专用凝结芽孢杆菌、嗜热乳酸杆菌、鼠李糖乳杆菌混合加入,组份及百分重量为:葡萄糖5%、牛肉膏2%...
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