The invention discloses a pelletizing process for removing heavy metals from yeast. The steps are as follows: after solid activation and liquid activation, yeast sludge is obtained by centrifugation after washing with sterile water; 0.5%5% sodium alginate solution is prepared, and ultrasonic exhaust is exhausted; the sodium alginate solution and yeast sludge are mixed evenly by mixer according to the volume ratio of 1_4:1; The mixed solution of sodium alginate and yeast mud was dripped into 0.5%-5% calcium chloride solution to granulate. The immobilized yeast pellets were placed in calcium chloride solution for 30 minutes and 2 hours, then filtered with gauze. Then the pellets were washed twice with distilled water to remove the residual calcium chloride solution. The immobilized microbial yeast pellets obtained by granulation have good removal effect of heavy metals, and the reasonable volume ratio of sodium alginate to yeast slurry is determined under the condition of determining the microbial embedding rate. It can not only ensure good embedding effect, but also reduce the dosage of sodium alginate or yeast slurry, so as to achieve the purpose of cost saving.
【技术实现步骤摘要】
脱除重金属酵母小球造粒工艺
本专利技术涉及一种脱除重金属酵母小球造粒工艺。
技术介绍
食品行业中工业化脱除食品物料中的超标重金属技术都是首先用酸对食品物料进行酸解,其次用酵母进行吸附重金属。包括用海藻酸钠-氯化钙对酵母进行包埋固定化处理,以便酵母菌与食品物料进行分离。已有技术可能操作起来成本较高,能耗较大。因为在工艺中要回收酸解后上清液中的有机物质,包括后续处理底部沉淀中的重金属,要将这些物质进行烘干或者冻干,能耗较大。
技术实现思路
本专利技术为实现上述目的所采用的技术方案是:脱除重金属酵母小球造粒工艺,步骤如下:将酵母菌,经过固体活化和液体活化并扩大培养之后,用无菌水清洗,离心得到酵母菌泥;配制0.5%-5%的海藻酸钠水溶液,超声排气;用搅拌器将海藻酸钠水溶液与酵母菌泥按1-4:1的体积比混匀;将匀混的海藻酸钠-酵母菌泥混合液滴入0.5%-5%的氯化钙溶液中造粒;制成的固定化酵母小球在氯化钙溶液中静置30min-2h,然后用纱布过滤,随后用蒸馏水将小球再进行二次清洗,洗去上面残余的氯化钙溶液。进一步地,所述海藻酸钠水溶液的浓度优选为2%。进一步地,所述海藻酸钠水溶 ...
【技术保护点】
1.脱除重金属酵母小球造粒工艺,其特征在于,步骤如下:将酵母菌,经过固体活化和液体活化并扩大培养之后,用无菌水清洗,离心得到酵母菌泥;配制0.5%‑5%的海藻酸钠水溶液,超声排气;用搅拌器将海藻酸钠水溶液与酵母菌泥按1‑4:1的体积比混匀;将匀混的海藻酸钠‑酵母菌泥混合液滴入0.5%‑5%的氯化钙溶液中造粒;制成的固定化酵母小球在氯化钙溶液中静置30min‑2h,然后用纱布过滤,随后用蒸馏水将小球再进行二次清洗,洗去上面残余的氯化钙溶液。
【技术特征摘要】
1.脱除重金属酵母小球造粒工艺,其特征在于,步骤如下:将酵母菌,经过固体活化和液体活化并扩大培养之后,用无菌水清洗,离心得到酵母菌泥;配制0.5%-5%的海藻酸钠水溶液,超声排气;用搅拌器将海藻酸钠水溶液与酵母菌泥按1-4:1的体积比混匀;将匀混的海藻酸钠-酵母菌泥混合液滴入0.5%-5%的氯化钙溶液中造粒;制成的固定化酵母小球在氯化钙溶液中静置30min-2h,然后用纱布过滤,随后用蒸馏水将小球再进行二...
【专利技术属性】
技术研发人员:徐莹,张丹丹,陈鹏,封福鲜,吴岩强,汪东风,
申请(专利权)人:中国海洋大学,大连棒棰岛海产股份有限公司,
类型:发明
国别省市:山东,37
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