The invention discloses a PEDV S RBD linear B cell epitope, two specific recognition monoclonal antibodies and their applications. The amino acid sequence of the B cell epitope is TIDLFGYP. The invention immunizes BALB/c mice with purified PEDV S RBD recombinant protein to obtain monoclonal antibodies; identifies two monoclonal antibodies 4D8F10 and 6F3E3 by IPMA; expresses different fragments of PEDV S RBD recombinant protein by HEK 293T recombinant eukaryotic cells, and specifically recognizes the C-terminal region of PEDV S RBD by two monoclonal antibodies identified by Dot blot. The sequence was TSLLASACTIDLFGYP, and the synthetic polypeptide in the C-terminal region of PEDV S RBD was further identified by ELISA. Two monoclonal antibody specific recognition sequences were identified as linear B-cell epitopes of TIDLFGYP. The PEDV S RBD linear B cell epitope identified by the invention enriches the immunological function of PEDV S RBD, provides a reference for the follow-up study of S protein antigen drift, and can be applied to the development of antiviral drugs.
【技术实现步骤摘要】
一种PEDVS-RBD线性B细胞表位及两株特异性识别单克隆抗体和应用
本专利技术涉及病毒学、分子生物学和免疫学领域，特别是涉及猪流行性腹泻病毒(porcineepidemicdiarrheavirus，PEDV)纤突糖蛋白(spikeglycoprotein，S)受体结合区(receptor-bindingdomain，RBD)线性B细胞表位及两株特异性识别单克隆抗体和应用。
技术介绍
猪流行性腹泻(porcineepidemicdiarrhea，PED)是由猪流行性腹泻病毒(PEDvirus，PEDV)引起的一种高度接触性猪肠道传染病，以发病猪呕吐、水样腹泻、脱水为主要特征。PED对各年龄阶段的猪都会造成影响，尤其对7日龄以内的哺乳仔猪具有高致死性，病死率高达100％。PED造成了我国养猪业巨大的经济损失，已成为制约我国养猪业健康发展的传染病之一。PEDV是一种具有囊膜的单股正链RNA病毒，属于套式病毒目(Nidovirales)冠状病毒科(Coronaviridae)α-型冠状病毒属(Alphacoronavirus)，具有冠状病毒特征性的囊膜“纤突”(spi...
【技术保护点】
1.一种PEDV S‑RBD线性B细胞表位，其特征在于，其氨基酸序列为：TIDLFGYP。
【技术特征摘要】
1.一种PEDVS-RBD线性B细胞表位，其特征在于，其氨基酸序列为：TIDLFGYP。2.特异性识别PEDVS-RBD线性B细胞表位的两株单克隆抗体4D8F10和6F3E3，其特征在于，特异性识别含有权利要求1所述PEDVS-RBD线性B细胞表位序列的多肽、蛋白及PEDV。3.一种权利要求1所述的PEDVS-RBD线性B细胞表位在制备PED及PEDV诊...
【专利技术属性】
技术研发人员：李睿，张改平，孙彦刚，乔松林，陈鑫鑫，郭振华，赵东，李学伍，
申请(专利权)人：河南省农业科学院，
类型：发明
国别省市：河南,41
还没有人留言评论。发表了对其他浏览者有用的留言会获得科技券。