The invention belongs to the field of plant genetic engineering, and specifically relates to the cloning and Breeding Application of the gene qSLWA9 for controlling the characters of horn length and grain weight of Brassica napus. A major QTL SLWA9 gene was cloned by map-based cloning. The nucleotide sequence of SLWA9 gene was shown as SEQ ID NO:1, the coding region of CDS gene was shown as SEQ ID NO:2, and the protein sequence of SLWA9 gene was shown as SEQ ID NO:3. This locus is located on chromosome A9 of Brassica napus and controls two yield traits of pod length and 1000-grain weight. The results showed that the expression level of qSLWA9 positively regulated the characters of pod length and grain weight in rapeseed. The qSLWA9 gene cloned by the invention can be applied to genetic improvement of Brassica napus. The invention also develops corresponding molecular marker primers, which can be used in molecular marker assisted breeding of Brassica napus.
【技术实现步骤摘要】
控制甘蓝型油菜角果长和粒重性状的基因qSLWA9的克隆与育种应用
本专利技术属于植物基因工程领域,具体涉及一种控制甘蓝型油菜角果长和粒重性状的基因qSLWA9的克隆与育种应用。本专利技术具体涉及控制甘蓝型油菜角果长和粒重性状的基因qSLWA9的分离克隆、功能验证及应用。
技术介绍
甘蓝型油菜(BrassicanapusL.)是一种被广泛种植的油料作物,其产量的提高是油菜育种中的重要目标。油菜的籽粒产量主要由单位面积有效角果数、每角果粒数和粒重三大因子构成,每个构成因子都在产量的提高中都发挥着重要作用(Chenetal.,2007)。这些产量相关性状都是典型的数量性状,由数量性状基因位点(QTL)控制。甘蓝型油菜是由二倍体的祖先种白菜(B.rapa)和甘蓝(B.oleracea)通过自然杂交并经历全基因组加倍而形成的异源四倍体物种(Chalhoubetal.,2014),基因组的复杂性增加了对产量相关性状QTL基因克隆的难度。目前,甘蓝型油菜产量相关性状的研究仍集中在QTL的定位工作上,成功通过图位克隆技术克隆到基因的报道还很少。因此,克隆产量相关性状的QTL基因, ...
【技术保护点】
1.一种控制甘蓝型油菜角果长、粒重性状的基因qSLWA9,其核苷酸序列如序列表SEQ ID NO:1所示。
【技术特征摘要】
1.一种控制甘蓝型油菜角果长、粒重性状的基因qSLWA9,其核苷酸序列如序列表SEQIDNO:1所示。2.如权利要求1所述的基因,该基因编码的蛋白质序列如SEQIDNO:2所示。3.权利要求1或2所述的基因qSLWA9在甘蓝型油菜改良和高产育种中的应用...
【专利技术属性】
技术研发人员:刘克德,王晶,石柳柳,宋居容,王博,
申请(专利权)人:华中农业大学,
类型:发明
国别省市:湖北,42
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