本发明专利技术涉及尿酸检测技术领域，特别涉及一种尿酸检测试剂，试剂R1中含有缓冲液，4-氨基安替比林，BSA，蔗糖，海藻糖，过氧化物酶，抗坏血酸氧化酶，胆红素氧化酶，烷基酚聚氧乙烯醚( APEO)，防腐剂；试剂R2中含有缓冲液，TOOS尿酸酶·BSA蔗糖·海藻糖烷基酚聚氧乙烯醚( APEO)，防腐剂。采用PIPES（哌嗪-1,4-二乙磺酸）缓冲液，添加稳定剂BSA，蔗糖，海藻糖，大大增强了试剂的稳定性；采用新型表面活性剂烷基酚聚氧乙烯醚( APEO)，不仅显著改善产品的测定性能，而且增强了试剂的稳定性和抗干扰能力。
【技术实现步骤摘要】
本专利技术涉及尿酸检测
，特别涉及一种尿酸检测试剂，还涉及使用此检测试剂的检测方法。
技术介绍
尿酸是嘌呤分解代谢的最终产物。由肾脏随尿液排出体外。健康成人体内尿酸含量约为1.1g，其中约15%存在于血液中，血液中尿酸经肾小球过滤后，大部分由肾小管重吸收。尿酸是血浆中非蛋白氮重要成分之一，在严重肾脏损害时，血中尿酸可显著升高，而轻度受损时变化不大。故血尿酸测定是诊断肾重度受损的敏感指标。目前，尿酸(UA)的检测方法有磷钨酸还原法、尿酸酶法和HPLC法。钨酸还原法是目前临床比较常用的方法，利用无蛋白滤液中的尿酸在碱性环境中可被磷钨酸氧化成尿囊素及二氧化碳，磷钨酸被还原成钨蓝(tungstenblue)，根据钨蓝颜色深浅，用710nm波长滤光板或红色滤片，与同样处理的标准比较，进行比色，以空白调零点，可求出其浓度值。该方法操作简单，但有溶血样本时不能用该法测试，且易受其他还原性物质干扰等缺点。HPLC检测法虽然简单，但样品预处理比较复杂，设备昂贵难以普及。而现在最流行的方法是尿酸酶-过氧化物酶偶联法，该法灵敏且不需要去蛋白，但容易受维生素C和胆红素等干扰物质的影响。鉴于此，本专利技术在尿酸酶-过氧化物酶偶联法的基础上，优化反应体系，采用PIPES缓冲液，添加多种抗干扰物质和稳定剂，显著改善了试剂的抗干扰能力和稳定性，是一种更加稳定、抗干扰能力强的尿酸（UA）试剂。
技术实现思路
本专利技术的目的是提供一种用于检测尿酸（UA）的试剂及使用该试剂检测尿酸含量的方法。该试剂盒采用尿酸酶-过氧化物酶偶联法，可以有效检测尿酸的含量，具有抗干扰能力强、稳定性好等优点。基本原理：尿酸在尿酸酶作用下，生成尿囊素和过氧化氢（H2O2），再利用Trinder反应系统，过氧化氢在过氧化物酶（POD）催化下，与4-氨基安替比林（4-AAP）和N-乙基-间甲苯胺丙磺酸钠（TOOS），生成红紫色色素。反应液的颜色与尿酸浓度成正比。。本专利技术是通过以下步骤得到的：一种尿酸检测试剂，包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下。试剂R1中含有：缓冲液···············································································100mmol/L4-氨基安替比林································································1.3mmol/LBSA···················································································1g/L蔗糖···················································································5g/L海藻糖···············································································2g/L过氧化物酶·······································································12KU/L抗坏血酸氧化酶·······························································5KU/L胆红素氧化酶···································································8KU/L烷基酚聚氧乙烯醚(APEO)·············································2g/L防腐剂················································································0.5g/L。试剂R2的组分为：缓冲液················································································100mmol/LTOOS··················································································13mmol/L尿酸酶·················································································2KU/LBSA·····················································································1g/L蔗糖·····················································································5g/L海藻糖·················································································2g/L烷基酚聚氧乙烯醚(APEO)···············································2g/L防腐剂··················································································0.5g/L。所述的尿酸检测试剂，试剂R1中缓冲液为25℃，pH为7.6的哌嗪-1,4-二乙磺酸缓冲液。所述的尿酸检测试剂，试剂R2中缓冲液为25℃，pH为7.6的哌嗪-1,4-二乙磺酸缓冲液。所述的尿酸检测试剂，所述防腐剂为NaN3。所述的尿酸检测试剂检测尿酸的检测方法，使用全自动生化分析仪利用终点法进行测定，检测主波长为546nm。所述的检测方法，R1试剂和R2试剂的比例为4:1。本专利技术的有益效果：1）采用新的缓冲体系和稳定剂，显著改善了试剂的稳定性；2）采用新型表面活性剂烷基酚聚氧乙烯醚(APEO)，不仅显著改善测定的性能，而且增强了试剂的稳定性和抗干扰能力；3）试剂的准确度和稳定性良好，价格便宜，使用方便，完全可以满足临床需要。附图说明图1为两种试剂的相关性曲线图；图2为两种试剂效期稳定性曲线图。具体实施方式下面结合具体实施例对本专利技术进行进一步说明。实施例1：尿酸的检测试剂，包括试剂R1和试剂R2。其R1的组成为：PIPES（哌嗪-1,4-二乙磺酸）缓冲液(pH=7.6，25℃)····························100mmol/L4-氨基安替比林······················································································本文档来自技高网...
【技术保护点】
一种尿酸检测试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：1)试剂R1的组分为：缓冲液···············································································100mmol/L4‑氨基安替比林································································1.3mmol/LBSA···················································································1g/L蔗糖···················································································5g/L海藻糖···············································································2g/L过氧化物酶·······································································12KU/L抗坏血酸氧化酶·······························································5KU/L胆红素氧化酶···································································8KU/L烷基酚聚氧乙烯醚(APEO)·············································2g/L防腐剂················································································0.5g/L2)试剂R2的组分为：缓冲液···············································································100mmol/LTOOS·················································································13mmol/L尿酸酶················································································2KU/LBSA····················································································1g/L蔗糖····················································································5g/L海藻糖················································································2g/L烷基酚聚氧乙烯醚(APEO)··············································2g/L防腐剂·················································································0.5g/L...
【技术特征摘要】
1.一种尿酸检测试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：1)试剂R1的组分为：缓冲液···············································································100mmol/L4-氨基安替比林································································1.3mmol/LBSA···················································································1g/L蔗糖···················································································5g/L海藻糖···············································································2g/L过氧化物酶·······································································12KU/L抗坏血酸氧化酶·······························································5KU/L胆红素氧化酶···································································8KU/L烷基酚聚氧乙烯醚(APEO)·············································2g/L防腐剂················································································0.5g/L2)试剂R2的组分为：缓冲液······························...
【专利技术属性】
技术研发人员：谭伯清，李志明，甘宜梧，肖慧，王绮，
申请(专利权)人：章丘美高义医疗器械有限公司，
类型：发明
国别省市：山东;37
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