The invention relates to a method for constructing a genuine medicinal herb research model, in particular a hairy root model and an application thereof, so as to provide an experimental platform for the study of the biological causes of the genuine medicinal herbs. In the case of genuine Danshen, which comprises the comparison between the hairy root and raw material similarity, stability, confirm the hairy roots of nuclear DNA confirm the stability of genetic stability and chemical composition of the rol gene. The simulation also includes environmental factors, namely, by changing the conditions of the hairy root growth and the content of effective components of culture, determine the phenotypic variation under environmental conditions; simulation of genetic factors, namely through the changes of genetic material of hairy roots, or the use of hairy roots of different genotypes of Salvia miltiorrhiza on the growth status and content of effective components change, determine the genetic mechanism of phenotypic variation. The model has the characteristics of similarity, repeatability, openness, ease of use, combination of quantitative and qualitative characteristics. It can be used to study the biological characteristics of the genuine Salvia miltiorrhiza bge.
【技术实现步骤摘要】
【技术保护点】
一种用于道地药材研究的毛状根模型构建方法，其特征在于包括下列步骤： （１）比较毛状根和其原药材之间的相似性：①对毛状根和药材中的指标成分进行定量比较；②进行毛状根和药材指纹图谱的相似度分析；③毛状根毒性试验；④从整体、器官以及细胞三个 水平上观察毛状根和药材的药理作用； （２）随机选取不少于５个批次的毛状根材料，确认毛状根核ＤＮＡ的稳定性； （３）随机选取不少于１０个批次的毛状根材料，确定ｒｏｌ基因的遗传稳定性，分析其化学成分的稳定性； （４）模拟环境因 子，通过改变毛状根培养条件研究其生长量和有效成分含量的变化，确定环境条件下的...
【技术特征摘要】
【专利技术属性】
技术研发人员：黄璐琦，袁媛，吕冬梅，梁日欣，
申请(专利权)人：中国中医科学院中药研究所，
类型：发明
国别省市：11[中国|北京]
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