一种以混合筛分介质修饰DNA微流控芯片微通道的方法技术

一种以混合筛分介质修饰ＤＮＡ微流控芯片微通道的方法，属ＤＮＡ微流控芯片电泳分析的技术领域。先配制聚乙烯吡咯烷酮与羟乙基纤维素的混合筛分介质作为修饰剂，再用修饰剂对ＤＮＡ微流控芯片微通道进行修饰。有以下优点：操作步骤简便易行；微通道经本发明专利技术的方法修饰的ＤＮＡ微流控芯片作ＤＮＡ片段的分析时，对生化样品ＤＮＡ的筛分效果好，分析时间短，修饰剂的使用寿命较长。特别适于用来对ＤＮＡ微流控芯片的微通道进行修饰，使被修饰的芯片成为高效优质的ＤＮＡ电泳分析器具。

Method for modifying micro channel of DNA microfluidic chip by mixed sieving medium

The invention relates to a method for modifying a microchannel of a DNA microfluidic chip by a mixed sieving medium, belonging to the technical field of electrophoretic analysis of DNA microfluidic chips. Firstly, the mixed sieving medium of polyvinylpyrrolidone and hydroxyethyl cellulose was used as modifier, and the micro channel of DNA microfluidic chip was modified with modifier. Has the following advantages: simple operation steps; micro channel via the method of the present invention modified DNA microfluidic chip for DNA fragment analysis, screening effect on biochemical samples DNA, short analysis time, long service life modifier. The utility model is especially suitable for modifying micro channels of DNA microfluidic chips so as to make the modified chips into high efficiency and high quality DNA electrophoresis analysis instruments.

【技术实现步骤摘要】

【技术保护点】
一种以混合筛分介质修饰ＤＮＡ微流控芯片微通道的方法，其特征在于，具体操作步骤：　第一步配制修饰剂　以聚乙烯吡咯烷酮与羟乙基纤维素的混合筛分介质作为修饰剂，将２．８ｍｇ～８．４ｍｇ的聚乙烯吡咯烷酮和２．８ｍｇ～８．４ｍｇ羟乙基纤维 素溶解于２．８ｍＬ的ｐＨ　９．２的三羟甲基氨基甲烷－硼酸－ＥＤＴＡ溶液中，聚乙烯吡咯烷酮∶羟乙基纤维素的质量比为１∶１，超声搅拌，驱除溶液中的气泡，得到质量浓度为１％～３％的聚乙烯吡咯烷酮与羟乙基纤维素的混合溶液即修饰剂，经０．４５μｍ微 孔滤膜过滤，放入４℃的冰箱备用；　第二步ＤＮＡ微流控芯片及其微通道的预处理　先将ＤＮＡ微流控芯片及其微通道...

【技术特征摘要】

【专利技术属性】
技术研发人员：李钢，葛淑丽，王清江，何品刚，方禹之，
申请(专利权)人：华东师范大学，
类型：发明
国别省市：31[]