circ_1892及其在制备鼻咽癌治疗制剂中的应用和治疗制剂制造技术

本发明专利技术属于肿瘤分子生物学技术领域，具体涉及circ_1892及其在制备鼻咽癌治疗制剂中的应用和治疗制剂。本发明专利技术通过构建过表达载体在鼻咽癌细胞系HONE1、HNE2和CNE2中确保circ_1892被过表达后，进行Transwell小室实验及划痕愈合实验，相对于对照组，过表达组细胞的侵袭与迁移速度明显减慢；相反地，通过RNA干扰技术在鼻咽癌细胞系HONE1、HNE2和CNE2中下调circ_1892的表达后，相对于对照组，细胞的侵袭与迁移速度明显加快。即circ_1892可以抑制鼻咽癌细胞的侵袭迁移。表明过表达circ_1892能延缓鼻咽癌扩散转移，具有深远的临床意义和重要的推广应用前景。

Circ_1892 and its application in the preparation of nasopharyngeal carcinoma therapeutic preparations

The invention belongs to the technical field of tumor molecular biology, in particular to circ_1892 and its application in the preparation of nasopharyngeal cancer therapeutic preparations and therapeutic preparations. By constructing an overexpression vector to ensure the overexpression of circ_1892 in nasopharyngeal carcinoma cell lines HONE1, HNE2 and CNE2, the Transwell chamber experiment and scratch healing experiment were carried out. Compared with the control group, the invasion and migration speed of the overexpression group cells was significantly slowed down. On the contrary, the expression of circ_1892 in nasopharyngeal carcinoma cell lines HONE1, HNE2 and CNE2 was down-regulated by RNA interference technology. Compared with the control group, the rate of cell invasion and migration was significantly faster. That is, circ_1892 can inhibit the invasion and migration of nasopharyngeal carcinoma cells. It is concluded that overexpression of circ_1892 can delay the spread and metastasis of nasopharyngeal carcinoma, and has far-reaching clinical significance and important application prospects.

【技术实现步骤摘要】
circ_1892及其在制备鼻咽癌治疗制剂中的应用和治疗制剂
本专利技术属于肿瘤分子生物学
，具体涉及一种环状RNAcirc_1892，以及过表达环状RNAcirc_1892的试剂和其在制备鼻咽癌治疗制剂中的应用。
技术介绍
目前环状RNA(CircularRNA)是个研究热点，circRNA主要来源于蛋白质编码基因的外显子区，也可以由内含子区、UTR区、基因间区、非编码RNA位点及已知转录物的反义位点形成。CircRNA是由前体mRNA(precursormessengerRNA,pre-mRNA)反向拼接而形成的一类不具有5‘末端帽子和3’末端poly(A)尾巴并以共价键形成环形结构的非编码RNA分子。CircRNA形成过程可以分为两大类，即外显子环化(exoncircularization)和内含子环化(introncircularization)两大机制。Jeck等提出外显子来源的circRNA(exoniccircRNA,ecircRNA)可以分为套索驱动环化(lariat-drivencircularization)和内含子配对驱动环化(intron-pairing本文档来自技高网...

【技术保护点】
1.一种环状RNA circ_1892，序列如SEQ ID NO.1所示。

【技术特征摘要】
1.一种环状RNAcirc_1892，序列如SEQIDNO.1所示。2.过表达环状RNAcirc_1892的试剂在制备治疗鼻咽癌制剂中的应用，所述的环状RNAcirc_1892序列如SEQIDNO.1所示。3.根据权利要求2所述的应用，其特征在于，所述的过表达环状RNAcirc_1892的试剂包括过表达环状RNAcirc_1892的质粒载体。4.根据权利要求3所述的应用，其特征在于，所述的过表达环状RNAcirc_1892的质粒载体利用pcDNA3.1质粒载体构建。5.根据权利要求3所述的应用，其特征在于，所述的过表达环状RNAcirc_1892的质粒载体构建时的酶切位点为Sacll和Clal。6.一种治疗鼻咽癌的制剂，其特征在于，包括过表达环状R...

【专利技术属性】
技术研发人员：熊炜，曾朝阳，郭灿，范春梅，赵梦瑶，熊芳，王忆安，莫勇真，张文玲，周鸣，李小玲，李桂源，
申请(专利权)人：中南大学，
类型：发明
国别省市：湖南,43