The invention discloses a house dust mite allergen Der P 22 gene recombinant protein and its application. The Der P 22 gene of house dust mite allergen is shown in SEQ ID NO:2. The recombinant protein is expressed and purified by Der P 22 gene of house dust mite allergen, and the sequence is shown in SEQ ID NO:1. The recombinant protein is used to prepare diagnostic reagents or biological products for accurate medical detection of allergic diseases caused by house dust mites. The full length of Der P 22 gene sequence of house dust mite was found for the first time, and its recombinant protein was obtained by gene cloning, expression and purification. The recombinant protein was used to accurately detect 16 children with allergic asthma caused by house dust mite in Western blotting experiment, and the positive rate was 93.75%, while the control group was not.
【技术实现步骤摘要】
一种屋尘螨过敏原Derp22基因重组蛋白及其应用
本专利技术属于变态反应学领域，尤其涉及一种屋尘螨过敏原Derp22基因重组蛋白及其在制备精准医学检测屋尘螨引起的过敏性疾病的诊断试剂或生物制品方面的应用。
技术介绍
尘螨(housedustmites)指孳生于居室地毯、床、纺织品和家具等处积尘中的螨类，文献报道多达100种以上。世界卫生组织(WorldHealthOrganization,WHO)和国际免疫学会联盟(InternationalUnionofImmunologicalSocieties，IUIS)授权的过敏原命名委员会网站(http://www.allergen.org/)已公布了粗脚粉螨(Acarussiro)、热带无爪螨(Blomiatropicalis)、粉尘螨(Dermatophagoidesfarinae)、微角尘螨(Dermatophagoidesmicroceras)、屋尘螨(Dermatophagoidespteronyssinus)、梅氏嗜霉螨(Euroglyphusmaynei)、家食甜螨(Glycyphagusdomesticu...
【技术保护点】
1.一种屋尘螨过敏原Derp 22基因重组蛋白，其特征在于，该蛋白序列如SEQ ID NO：1所示。
【技术特征摘要】
1.一种屋尘螨过敏原Derp22基因重组蛋白，其特征在于，该蛋白序列如SEQIDNO：1所示。2.权利要求1所述的屋尘螨过敏原Derp22基因重组蛋白在制备精准医学检测屋尘螨引起的过敏性疾病的诊断试剂或生物制品方...
【专利技术属性】
技术研发人员：崔玉宝，郁志伟，钱俊，杜晓燕，陈越新，吴国荣，周鹰，
申请(专利权)人：无锡市人民医院，
类型：发明
国别省市：江苏,32
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