The invention discloses a polysaccharide and its application in CIK cell culture. The preparation methods include: soaking the leaves of Robinia pseudoacacia with 95% ethanol, filtering and collecting filter residue; extracting filter residue with water, filtering and collecting filtrate; adding 95% ethanol to filtrate, stationary and centrifugal collecting and precipitating; dissolving precipitation water, freezing and drying to obtain purple. The crude polysaccharide of Sophora japonica leaves was prepared into 1% polysaccharide solution, which was slowly dripped with anhydrous ethanol to ethanol concentration of 30%. The supernatant was slowly dripped with anhydrous ethanol to ethanol concentration of 40%. After centrifugation, the supernatant was slowly dripped with anhydrous ethanol to ethanol concentration of 40%. After centrifugation, the supernatant was dripped slowly. Adding absolute ethanol to ethanol concentration up to 50%, static centrifugation, precipitation and re-dissolution freeze-drying F50 components; supernatant slowly dripping with absolute ethanol to ethanol concentration up to 60%, static centrifugation, precipitation and re-dissolution freeze-drying F60 components.
【技术实现步骤摘要】
一种多糖及在CIK细胞培养方面的应用
本专利技术属于化学领域，涉及一种多糖及在CIK细胞培养方面的应用。
技术介绍
紫穗槐Amorphafruticosa为豆科Leguminosae多年生落叶灌木，别名棉槐、椒条、棉条、穗花槐。花，清热、凉血、止血；叶，微苦、凉，具有祛湿消肿功效，主治痈肿、湿疹、烧烫伤。原产美国东北部和东南部，系多年生优良绿肥，蜜源植物，耐瘠，耐水湿和轻度盐碱土，又能固氮。现我国东北、华北、西北及山东、安徽、江苏、河南、湖北、广西、四川等省区均有栽培。枝叶作绿肥、家畜饲料；茎皮可提取栲胶，枝条编制篓筐；果实含芳香油，种子含油率10％，可作油漆、甘油和润滑油之原料。栽植于河岸、河堤、沙地、山坡及铁路沿线，有护堤防沙、防风固沙的作用。近年来，对紫穗槐的化学成分和药理活性研究的逐步深入，发现很多活性成分，在抗肿瘤、抗病毒、保肝、杀虫等方面显示出潜在的药用价值。
技术实现思路
本专利技术的目的在于提供一种多糖及在CIK细胞培养方面的应用。本专利技术通过下面的技术方案实现：技术方案一：一种用于促进CIK细胞增殖的多糖，重均分子量为322.3KDa。上述多糖的制...
【技术保护点】
1.一种用于促进CIK细胞增殖的多糖，重均分子量为258.1KDa。
【技术特征摘要】
1.一种用于促进CIK细胞增殖的多糖，重均分子量为258.1KDa。2.权利要求1所述多糖的制备方法，其特征在于，包括如下步骤：步骤S1、紫穗槐叶粗多糖的制备将紫穗槐叶剪碎，用体积分数95％乙醇溶液浸泡24h，料液比1:40，过滤收集滤渣；滤渣用水于85℃提取3次，每次2h，料液比1:25，合并提取液，过滤收集滤液；滤液真空浓缩至原有体积1/5，加入5倍体积95％乙醇，静置24h，离心收集沉淀；沉淀用水复溶，冷冻干燥得到紫穗槐叶粗多糖；步骤S2、紫穗槐叶分级多糖F40的制备每10g紫穗槐...
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