Culture medium for Vibrio BS02 and preparation method thereof. The invention relates to a culture medium for microorganisms, and provides a culture medium suitable for culturing Vibrio BS02, which can significantly increase the cell biomass and the production of the algae killing active substances. The peptone, yeast powder, glucose, NaCl, MgSO4, 7H2O, KCl, K2HPO4, 3H2O, CaCl2, 2H2O and Fe2 (SO4) 3 were added into distilled water and fixed to 1L. After sterilization, the culture medium of Vibrio BS02 was obtained. As basic medium LB, investigated the effect of medium composition and amount of Vibrio BS02 in shake flask, and the use of Plackett-Burman (PB) design method and response surface optimization design were conducted to optimize the culture medium, developed a culture of Vibrio BS02 suitable for high density composite medium.
【技术实现步骤摘要】
本专利技术涉及微生物培养基，尤其涉及一种弧菌BS02的培养基及其制备方法。
技术介绍
随着现代工业化的快速发展和人口的增多，大量的工农业废水和生活污水不经任何处理直接排放到海洋中，引起海洋水体富营养化加剧，赤潮频繁发生(1、Hallegraeff GM. A reviewof harmful algal blooms and their apparent global increase. Phycologia. 1993,32 :79-99)。有害赤潮已成为全球性的海洋灾害，严重制约了沿海经济的发展，破坏海洋生态环境和威胁人类健康，因而如何有效的防治赤潮成为当前国内外急需解决的难题。在海洋生态系统中，微生物在海洋生物多样性、海洋生物丰度及海洋循环系统中占据重要的地位。海洋微生物在赤潮生消的过程中所扮演的重要角色也越来越受到相关研究人员的关注。研究报道较多的是杀藻细菌通过分泌胞外杀藻活性物质杀藻，细菌可以通过分泌胞外物质，如蛋白质、多肽、氨基酸、抗生素、羟胺和含氮化合物等杀死藻细胞 (2、Amaro AM, Fuentes MS, Ogalde SR, et a...
【技术保护点】
１．一种弧菌ＢＳ０２的培养基，其特征在于所述弧菌ＢＳ０２已于２０１０年０９月０３日保藏于中国典型培养物保藏中心，保藏中心保藏编号为ＣＣＴＣＣ ＮＯ：Ｍ２０１０２１７；所述弧菌ＢＳ０２的培养基的组成是在１Ｌ的蒸馏水中含有：胰蛋白胨１．５～２．７ｇ；酵母粉 ０．７５～２．２５ｇ；葡萄糖 ０．５～１．０ｇ；ＮａＣｌ ２０～３０ｇ，ＭｇＳＯ４·７Ｈ２Ｏ ０．１～０．３ｇ；ＫＣｌ１～３ｇ；ＫＢｒ ０．１～０．５ｇ；Ｋ２ＨＰＯ４·３Ｈ２Ｏ ０．０７～０．２１ｇ；ＣａＣｌ２·２Ｈ２Ｏ ...
【技术特征摘要】
【专利技术属性】
技术研发人员：郑天凌，安新丽，傅丽君，田蕴，
申请(专利权)人：厦门大学，
类型：发明
国别省市：92
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