一种辅助筛选耐盐大豆的方法及其专用引物技术

本发明专利技术公开了一种辅助筛选耐盐大豆的方法及其专用引物。本发明专利技术提供的专用引物是序列表的序列３和序列表的序列４所示核苷酸组成的特异性引物对。本发明专利技术提供的方法包括如下步骤：以待测大豆的基因组ＤＮＡ为模板，用所述特异性引物对进行ＰＣＲ扩增，检测扩增产物，扩增产物中具有３８３ｂｐ片段的待测大豆为耐盐大豆，扩增产物中具有２６３ｂｐ片段且不具有３８３ｂｐ片段的待测大豆为盐敏感大豆。应用本发明专利技术的特异性引物对辅助筛选耐盐大豆或盐敏感大豆，操作简便、正确率高。本发明专利技术可以应用于大豆耐盐性状的鉴定，可以进行早期筛选。将本发明专利技术的方法和特异性引物对进行育种，能够缩短育种时间，节约育种成本，增加育成成功率，具有重大实际价值。

Method for assisting screening salt tolerant soybean and its special primer

The invention discloses a method for assisting screening salt tolerant soybean and a special primer thereof. A specific primer provided by the invention is a sequence of Table 3 and a sequence of Table 4, wherein a specific primer pair is composed of nucleotides. The method provided by the invention comprises the following steps: with the test of soybean genomic DNA as template, amplification of PCR with the specific primers, PCR products and PCR products with 383bp fragment to be tested for salt tolerant soybean soybean, the PCR products with 263bp fragments and do not have to be tested for 383bp fragment of soybean salt sensitive soybean. The specific primers of the present invention are useful for screening salt tolerant soybean or salt sensitive soybean, and the operation is simple and the accuracy is high. The invention can be applied to the identification of salt tolerance in soybean and can be screened early. By breeding the method and the specific primer pair, the breeding time can be shortened, the breeding cost can be saved, and the success rate of breeding can be increased, and the utility model has great practical value.

【技术实现步骤摘要】

【技术保护点】
１．由序列表的序列３和序列表的序列４所示核苷酸组成的特异性引物对。

【技术特征摘要】

【专利技术属性】
技术研发人员：关荣霞，邱丽娟，刘光宇，常汝镇，田蕾，
申请(专利权)人：中国农业科学院作物科学研究所，
类型：发明
国别省市：11[中国|北京]