一种含多肽链段的杂化涂层的制备方法技术
Method for preparing hybrid coating containing polypeptide chain
The present invention provides hybrid coating preparation method of a polypeptide chain containing the polypeptide chain grafted polyelectrolyte through preparation, configuration of polyelectrolyte solution, using LbL technology, construction of organic / inorganic hybrid polyelectrolyte coating on the surface of implant with collagen and hydroxyapatite nanoparticles with hyaluronic acid and chondroitin sulfate, get hybrid RGD containing polyelectrolyte composite coating. Because nano hydroxyapatite particles can absorb in the body faster, therefore, in vivo showed hydroxyapatite coating can on the regeneration of bone tissue and activity, can be absorbed in the bone tissue reconstruction is completed; at the same time, the implant material containing RGD hybrid coatings, the initial adhesion will promote bone cells on the surface of implant; then. Can promote bone cell differentiation, proliferation and extracellular matrix; finally, with the active coating absorbed by human body, achieve the reconstruction of new bone tissue on the implant surface.
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【技术保护点】
一种含多肽链段的杂化涂层的制备方法，其特征在于，通过以下步骤实现：（１）制备多肽链段接枝的聚电解质：先将ＧＲＧＤＳＰ多肽链段和聚电解质溶解在水溶液中配置成混合溶液，然后加入碳化二亚胺／Ｎ－羟基琥珀酰亚胺催化体系，室温下反应４小时，然后将所得到的产物在纯净水中透析２周，冻干，得到多肽链段接枝的聚电解质；其中：所述聚电解质选用透明质酸、硫酸软骨素，得到的产物是多肽链段接枝的透明质酸或多肽链段接枝的硫酸软骨素，透明质酸和硫酸软骨素的分子量为１００－５００００００Ｄ；（２）配置聚电解质溶液：将步骤（１）获得的多肽链段接枝的聚电解质用ＮａＣｌ溶液配置成溶液，作为聚阴离子电解质溶液；将...
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0来自[北京市联通] 2015年03月09日 22:11细胞核中DNA的某一区段转录出来的mRNA从核孔穿出来进入细胞质中，与核糖体结合起来。蛋白质合成就在核糖体进行。蛋白质开始合成时，首先核糖体与mRNA结合在一起，核糖体附着在mRNA的一端（起动部位），然后沿着mRNA从5′3′方向移动（当核糖体向前移动不久，另一个核糖体又结合上去，所以一个mRNA可以有多个核糖体连续上去）。