添加金属离子提高灰绿霉素Ａ产量的方法技术

一种通过添加金属离子提高灰绿霉素Ａ产量的方法，属于代谢调控优化发酵过程技术领域。本发明专利技术是利用海洋丝状真菌灰绿曲霉ＨＢ１－１９（Ａｓｐｅｒｇｉｌｌｕｓ？ｇｌａｕｃｕｓ？ＨＢ１－１９）ＣＣＴＣＣ？Ｍ？２０６０２２为生产菌株，通过在两种不同的发酵培养基中添加氯化镍、氯化钴及六水合硝酸钻来提高灰绿霉素Ａ的产量。其中在发酵培养基Ａ中最高产量可达到２４．３ｍｇ／ｌ，是不加金属离子的对照组提高了６７％；在发酵培养基Ｂ中最高产量可达到８１．１ｍｇ／ｌ，是不加金属离子的对照组提高了４５％。该发明专利技术对于海洋真菌灰绿曲霉ＨＢ１－１９发酵研究及灰绿霉素Ａ代谢调控研究具有重要意义。

Method for adding metal ion to increase production of lime green fungus A

The invention relates to a method for improving the yield of green ash A by adding metal ions, which belongs to the technical field of metabolic regulation and optimization of fermentation process. The present invention utilizes marine filamentous fungus Aspergillus niger HB1 - 19 (Aspergillus? Glaucus? HB119) CCTCC? M? 206022 as a production strain, the yield of lincomycin A was increased by adding nickel chloride, cobalt chloride and six hydrated nitric acid in two different fermentation mediums. The highest yield in fermentation culture medium A reached 24.3mg / L, the control group without metal ions increased by 67%; the highest yield in the fermentation culture medium B reached 81.1mg / L, the control group without metal ions increased by 45%. The invention is of great significance for the study of fermentation of marine fungi, Botrytis and HB1 19, and the regulation of the metabolism of Botrytis A.

【技术实现步骤摘要】

【技术保护点】
一种通过添加金属离子提高灰绿霉素Ａ产量的方法，其特征是利用海洋丝状真菌灰绿曲霉ＨＢ１－１９ＣＣＴＣＣＭ２０６０２２为生产菌株，在发酵培养基中添加钴离子或镍离子化合物中的至少一种来提高灰绿霉素Ａ的产量。

【技术特征摘要】

【专利技术属性】
技术研发人员：周祥山，张元兴，孙学谦，
申请(专利权)人：华东理工大学，
类型：发明
国别省市：31[中国|上海]